河南城建学院学报2025，Vol.34Issue(2)：114-120,7.DOI:10.14140/j.cnki.hncjxb.2025.02.015
猪博卡病毒G1基因群PCR检测方法的建立及应用
Establishment and application of PCR assay for detection of Porcine Bocavirus Genogroup G1
摘要
Abstract
To establish a PCR assay for rapid detection of Porcine Bocavirus Genogroup G1(PBoV G1),a pair of specific primers were designed based on the conserved sequence of VP1 gene from the newly available PBoV G1 strains in GenBank.By optimizing the PCR amplification conditions and system,a PCR assay for the detec-tion of PBoV G1 was established.The specificity and sensitivity of this detection method were verified,and pre-liminary clinical application was carried out.The results of the specificity test show that this assay is exclusive-ly specific to PBoV G1,amplifying a distinct target band,while showing no cross-reactivity with other common porcine enteric viruses.The results of the sensitivity test show that the limit of detection for the PBoV G1 stand-ard positive plasmid is 5.46 × 103 copies/μL.Among 463 porcine diarrhea samples tested by the developed as-say,PBoV G1 is detected in 116 cases,yielding a positive rate of 25.05％.It indicates that the PCR assay es-tablished by this research institute has strong specificity and high sensitivity,and is of great significance for the pathogen detection,epidemic prevention and control,and epidemiological investigation of PBoV G1 in pigs.关键词
猪博卡病毒/G1基因群/PCR方法/VP1基因Key words
Porcine Bocavirus/Genogroup G1/PCR assay/VP1 gene分类
农业科技引用本文复制引用
温悦,张卓威,高定焯,赵宇琛,张亚,刘琪,刘昱涵,付朋飞..猪博卡病毒G1基因群PCR检测方法的建立及应用[J].河南城建学院学报,2025,34(2):114-120,7.基金项目
河南城建学院2024年大学生创新创业训练计划资助项目(202411765035) （202411765035）
