河南农业科学2025，Vol.54Issue(3)：77-86,10.DOI:10.15933/j.cnki.1004-3268.2025.03.009
桑树内生菌的分离鉴定及功能菌筛选
Identification and Functional Characterization of Endophytic Microbes from Mulberry Tree(Morus alba L.)
摘要
Abstract
This study used methods such as microbial culture,target gene sequence amplification,and homology identification to isolate and identify endophytic microbes in Morus alba L.,and explored the strain types,distribution characteristics,and functional characteristics of endophytic bacteria and fungi in the roots,stems,and leaves of Yunsang No.6 mulberry.The results showed that a total of 43 endophytic bacteria belonging to 9 genera and 22 endophytic fungi belonging to 14 genera were isolated from mulberry trees.Among them,21 bacteria and 11 fungi were isolated from roots,13 bacteria and 5 fungi were isolated from stems,and 9 bacteria and 6 fungi were isolated from leaves.There were significant differences in the distribution of endophytic microbes in the roots,stems,and leaves of mulberry trees,there were Falsibacillus pallidus,Bacillus velezensis and Pseudomonas koreensis shared in the roots and stems of mulberry trees,Pantoea agglomerans,Bacillus subtilis and Pseudomonas koreensis shared in the stems and leaves,and Pseudomonas koreensis shared in the roots,stems,and leaves;Endophytic fungi Colletotrichum siamense was shared in mulberry roots and leaves,but no common fungi were found to exist in the stems and leaves,stems and roots.Further screening of functional microbes showed that 8 strains of nitrogen fixing microbes(7 bacteria,1 fungus),4 strains of phosphorus solubilizing microbes(2 bacteria,2 fungi),and 8 strains of potassium solubilizing microbes(6 bacteria,2 fungi)were obtained from 65 endophytic microbes,respectively.In summary,endophytic bacteria and fungi in mulberry trees exhibit diversity and distribution differences in roots,stems,and leaves.Among endophytic bacteria and fungi in mulberry trees,there are functional strains that can dissolve phosphorus,nitrogen,and potassium.关键词
桑树/内生真菌/内生细菌/分离鉴定/功能菌Key words
Morus alba L./Endophytic fungi/Endophytic bacteria/Isolation and identification/Functional microbes分类
农业科技引用本文复制引用
李莎,杨加虎,王艳辉,黄新球,杨文..桑树内生菌的分离鉴定及功能菌筛选[J].河南农业科学,2025,54(3):77-86,10.基金项目
云南省农业联合基金面上项目(202301BD070001-223) （202301BD070001-223）
云南省农业科学院蚕蜂所2022年度青年创新基金项目(QC2022004) （QC2022004）
