河南农业科学2025，Vol.54Issue(3)：87-98,12.DOI:10.15933/j.cnki.1004-3268.2025.03.010
植物根际促生菌和褪黑素对大花金鸡菊抗氧化特性和砷吸收的影响
Effect of PGPR and Melatonin on the Antioxidant Properties and Arsenic Absorption of Coreopsis grandiflora
摘要
Abstract
Using Chaoyang seedlings of Coreopsis grandiflora as test materials,an indoor pot experiment was conducted to investigate the effects of inoculating plant growth promoting bacteria(PGPR)and foliar spraying of melatonin(MT)at concentrations of 5 μmol/L(MT1),20 μmol/L(MT2),and 50 μmol/L(MT3)under 80 mg/kg arsenic(As)stress on the growth,root architecture,chlorophyll content,chlorophyll fluorescence parameters,nutrient elements,As absorption,and antioxidant properties of C.grandiflora.The results showed that compared with the CK treatment without As stress,the overall plant height,leaf area,and total biomass(aboveground and root dry weight)of C.grandiflora were significantly reduced under As stress.Foliar spraying of different concentrations of MT and MT+PGPR treatment could alleviate the damage of As stress to C.grandiflora.Under As stress,compared with the treatment without PGPR inoculation or MT spraying,the root system configuration(total root length,total volume,total surface area,root tip number)of C.grandiflora treated with As+MT2+PGPR was significantly improved.The root activity increased by 166.1％,and the chlorophyll content,maximum photochemical efficiency(Fv/Fm)of PS Ⅱ,and photochemical quenching coefficient(qP)increased by 56.7％,15.6％,and 62.8％,respectively,while the non photochemical quenching coefficient(NPQ)decreased by 23.7％.As stress significantly reduced the nitrogen,phosphorus,and potassium content in the aboveground and root systems of C.grandiflora,while As+MT2+PGPR treatment could increase the nitrogen,phosphorus,and potassium content in the aboveground and root systems to varying degrees.At the same time,foliar spraying of different concentrations of MT and MT+PGPR could increase the As content in the aboveground and root parts of C.grandiflora,improve the As transport coefficient and enrichment coefficient,activate the activities of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),and glutathione reductase(GR),and increase the content of ascorbic acid(As A)and glutathione(GSH)to alleviate oxidative stress on C.grandiflora.In summary,As+MT2+PGPR treatment has the best effect on improving the resistance of C.grandiflora to As pollution.关键词
大花金鸡菊/植物根际促生菌/褪黑素/砷胁迫/根系构型/抗氧化酶Key words
Coreopsis grandiflora/Plant growth promoting rhizobacteria/Melatonin/Arsenic stress/Root architecture/Antioxidant enzyme分类
农业科技引用本文复制引用
梁亚男,邬梦晞,肖雍琴,曾鲸津,段益莉..植物根际促生菌和褪黑素对大花金鸡菊抗氧化特性和砷吸收的影响[J].河南农业科学,2025,54(3):87-98,12.基金项目
四川省教育厅2022-2024年职业教育人才培养和教育教学改革研究项目(GZJG2022-312) （GZJG2022-312）
