河南农业科学2025，Vol.54Issue(3)：120-128,9.DOI:10.15933/j.cnki.1004-3268.2025.03.013
牡丹PomiR2592bj及其靶基因PoSDR1的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of PomiR2592bj and Its Target Gene PoSDR1 of Tree Peony
摘要
Abstract
Based on the previous analysis of transciptome,miRNA and degradome of early-flowering Fengdan,early-flowering Fengdan mutant plants,and late-flowering Lianhe,we identified PomiR2592bj and its target gene PoSDR1,which were excavated to regulate the flowering period of tree peony.To further elucidate the biological characteristics and functions of PomiR2592bj and PoSDR1,we cloned a 113 bp sequence for PomiR2592bj and a 1 380 bp sequence for PoSDR1.Secondary structure analysis revealed that the precursor sequence of PomiR2592bj could form a stable stem-loop structure with mature sequences located at the 3'arm end;multiple sequence alignment indicated conservation within these mature sequences;phylogenetic tree analysis unveiled that the miR2592 family was subdivided into 10 branches with homology.The protein encoded by PoSDR1 contained 296 amino acids.The protein was hydrophilic with no transmembrane domain and signal peptide.19 Ser sites of PoSDR1 were phosphorylated,while 11 Thr sites were phosphorylated,and 2 Tyr sites were phosphorylated.PoSDR1 had a NADB_Rossmann superfamily conserved domain.PomiR2592bj was predicted to have a targeting relationship with PoSDR1.关键词
牡丹/PomiR2592bj/PoSDR1/克隆/生物信息学Key words
Tree peony/PomiR2592bj/PoSDR1/Cloning/Bioinformatics分类
农业科技引用本文复制引用
雷炀,高靖姗,南琼琼,郭丽丽,侯小改..牡丹PomiR2592bj及其靶基因PoSDR1的克隆与生物信息学分析[J].河南农业科学,2025,54(3):120-128,9.基金项目
国家自然科学基金项目(U1804233) （U1804233）
河南省高校科技创新人才项目(22HASTIT036) （22HASTIT036）
