河南农业科学2025，Vol.54Issue(3)：129-138,10.DOI:10.15933/j.cnki.1004-3268.2025.03.014
SP1/IGF2BP3轴对猪骨骼肌卫星细胞增殖的调控作用
The Regulatory Effect of the SP1/IGF2BP3 Axis on the Proliferation of Porcine Skeletal Muscle Satellite Cells
摘要
Abstract
This study investigated the effect of insulin-like growth factor 2 mRNA-binding protein 3(IGF2BP3)on the growth and development of porcine skeletal muscle and analyzed its biological function and expression regulation mechanism so as to provide a theoretical basis and candidate genes for genetic improvement in pigs.Porcine skeletal muscle satellite cells were utilized as the experimental material.RT-qPCR and Western blot were employed to measure the expression of cell proliferation marker genes PCNA、CDK4、Cyclin A2 and MKI67,and EdU and CCK-8 kits were used to detect cell proliferation viability and rate.The dual-luciferase assay of SP1 binding site mutations in the promoter region of the IGF2BP3 gene and the ChIP assay were employed to investigate the expression regulatory mechanism of the IGF2BP3 gene by the transcription factor SP1.The results showed that knocking down IGF2BP3 in porcine skeletal muscle satellite cells significantly upregulated the expression of cell proliferation marker genes,increased the proportion of EdU positive cells,and significantly increased the cell proliferation rate at 48 and 72 hours.The dual-luciferase activity was significantly reduced by mutations in the SP1 binding region,whereas the activity was significantly increased by SP1 overexpression.SP1 could bind to the promoter region of the IGF2BP3 gene.Interference with SP1 decreased IGF2BP3 expression,whereas overexpression of SP1 upregulated IGF2BP3 expression.Overexpression of SP1 partially eliminated the phenotype that knockdown of IGF2BP3 promoted proliferation of porcine skeletal muscle satellite cells.In conclusion,the transcription factor SP1 and the IGF2BP3 gene interact to regulate the proliferation of porcine skeletal muscle satellite cells.关键词
猪/骨骼肌/IGF2BP3/SP1/猪骨骼肌卫星细胞/增殖Key words
Pig/Skeletal muscle/IGF2BP3/SP1/Porcine skeletal muscle satellite cell/Proliferation分类
农业科技引用本文复制引用
黄雨馨,王伟,李一星,唐中林..SP1/IGF2BP3轴对猪骨骼肌卫星细胞增殖的调控作用[J].河南农业科学,2025,54(3):129-138,10.基金项目
广西科技计划项目(AB21196060) （AB21196060）
深圳市可持续发展专项(KCXFZ20201221173213037) （KCXFZ20201221173213037）
国家自然科学基金项目(U23A20229) （U23A20229）
巴马县人才科技计划项目(巴人科20210028) （巴人科20210028）
