吉林农业大学学报2025，Vol.47Issue(2)：216-225,10.DOI:10.13327/j.jjlau.2022.1674
盐胁迫对不同倍体水稻生理及抗氧化酶相关基因的影响
Effects of Salt Stress on Physiological and Antioxidant Enzyme-related Genes in Different Ploidy Levels of Rice
摘要
Abstract
After polyploidization,plants often exhibit higher levels of salt tolerance than their dip-loid parents.Diploid rice 93-11D and its homologous autotetraploid 93-11T were used as materials to measure the contents of proline(Pro),malondialdehyde(MDA),and soluble sugars,and the activi-ties of peroxidase(POD)and superoxide dismutase(SOD).The expression of genes related to SOD and POD was also detected to analyze the effects of the increased ploidy on salt resistance in rice.The phenotypic observation results showed that after 24 h of NaCl stress,both materials exhibited sig-nificant wilting,but the wilting degree of 93-11D was slightly greater than that of 93-11T.The physi-ological parameters of 93-11D and 93-11T after 24 h of NaCl stress showed that in the diploid,the content of Pro generally showed an upward trend(by 3.99%),the content of MDA and soluble sugar and the activity of POD generally showed a downward trend(by 2.93%,1.91%and 7.88%,respec-tively),and the activity of SOD generally showed an upward trend(by 2.91%),in the autotetraploid,the content of Pro generally showed a downward trend(by 8.96%),the contents of MDA and soluble sugars,and the activity of POD generally showed an upward trend(by 8.62%,0.77%,and 0.45%,re-spectively),and the activity of SOD generally showed an upward trend(by 17.58%).The gene expres-sion results showed that the expression levels of related genes in 93-11T were significantly increased compared to its diploid counterparts after 24 h of salt stress.The SOD and POD-related genes were closely associated with the antioxidant system.The upregulation of these genes in the autotetraploid rice led to enhanced antioxidant capacity,which may be one of the possible reasons why autotetraploid rice has a stronger ability to withstand salt stress compared to diploid rice.This study lays foundation for further exploration of the mechanisms underlying salt tolerance of diploid and autotetraploid rice.关键词
水稻/盐胁迫/染色体加倍/生理指标/抗氧化酶/基因表达Key words
rice/salt stress/chomosome doubling/physiological parameter/antioxidant enzyme/gene expression分类
农业科技引用本文复制引用
李德江,孔文昌,王薇,卢凤丹,吴雨洁,王宁宁,马建..盐胁迫对不同倍体水稻生理及抗氧化酶相关基因的影响[J].吉林农业大学学报,2025,47(2):216-225,10.基金项目
国家重点研发计划项目(2016YFD0102000),吉林省科技发展计划项目(20190301074NY),国家自然科学基金区域创新发展联合基金项目(U19A2027) （2016YFD0102000）
