吉林农业大学学报2025，Vol.47Issue(2)：245-253,9.DOI:10.13327/j.jjlau.2024.20388
3株芽孢杆菌的筛选、鉴定及其对人参锈腐病的生防作用
Screening and Identification of Three Strains of Bacillus and Their Biocontrol Effects on Ginseng Rust Rot Disease
摘要
Abstract
Targeting the pathogen of ginseng rust rot,Cylindrocarpon destructans,bacteria FS-MY3,MYW5 and MMW5 with antagonistic effect were screened from rhizosphere soil using gradient dilu-tion method and plate confrontation method,with inhibition rates ranging from 88.88%to 91.04%.These bacteria also showed strong inhibitory effects against Alternaria panax and Fusarium oxyspo-rum,with inhibition rates ranging from 77.67%to 85.89%and 87.60%to 88.77%,respectively.Based on morphological characteristics,physiological and biochemical properties,and 16S rDNA se-quence alignment analysis,strains FS-MY3 and MYW5 were identified as Bacillus subtilis and MMW5 as Bacillus amyloliquefaciens.The results showed that all the three strains of Bacillus amylo-liquefaciens had the ability to secrete protease,cellulase and iron carriers,and ginseng root disk ex-periment and pot experiments verified that the three strains of Bacillus amyloliquefaciens had good control effect on rust rot of ginseng.This study provides a theoretical basis for the biological control of ginseng diseases and the development of ginseng biocontrol bacterial agents.关键词
人参/人参锈腐病/生防菌/芽孢杆菌Key words
ginseng/ginseng rust rot/biocontrol strain/Bacillus分类
农业科技引用本文复制引用
何贵祥,苗馨月,战宇,周一,王二刚,陈长宝,李琼..3株芽孢杆菌的筛选、鉴定及其对人参锈腐病的生防作用[J].吉林农业大学学报,2025,47(2):245-253,9.基金项目
国家自然科学基金项目(82073969),吉林省重大科技专项(20200504003YY),吉林省自然科学基金项目(YDZJ202101ZYTS015),长春市科技发展计划项目(21ZGY13),长春中医药大学"杏林学者工程"青年科学家项目(QNKXJ2-2021ZR19) （82073969）
