吉林农业大学学报2025，Vol.47Issue(2)：264-272,9.DOI:10.13327/j.jjlau.2024.1192
高拮抗茄立枯丝核菌的生防暹罗芽孢杆菌N2发酵培养基及其发酵条件优化
Optimization of Fermentation Media and Conditions for Biocontrol Strain Bacillus siamensis N2 with High Antifungal Activity against Rhizoctonia solani
摘要
Abstract
Using Rhizoctonia solani as target pathogen,the optimal fermentation medium and shake flask fermentation conditions for the biocontrol bacterium Bacillus siamensis N2 were screened.Us-ing single factor level test and orthogonal test,the optimal fermentation medium formula and shake flask fermentation conditions were determined based on the growth rate and inhibitory rate of B.sia-mensis N2 against R.solani.The optimal fermentation medium formula for B.siamensis N2 is:glu-cose 20 g/L,peptone 50 g/L,glycerol 5 mL/L,K2HPO4·7H2O 1.5 g/L,and MgSO4·7H2O 1.5 g/L.The optimal conditions of shaker fermentation were:inoculation amount 4%,flask loading 100 mL/250 mL,culture time 24 h,culture temperature 28℃,rotation speed 180 r/min,and initial pH value 7.The study lays a theoretical foundation for the optimization of tank fermentation conditions and industrial production of N2 strain.关键词
暹罗芽孢杆菌/茄立枯丝核菌/发酵培养基/发酵条件Key words
Bacillus siamensis/Rhizoctonia solani/fermentation medium/fermentation conditions分类
农业科技引用本文复制引用
梅柏杨,李宇诗,孙国刚,邵郅伟,杨丽娜,卢宝慧,王雪,高洁..高拮抗茄立枯丝核菌的生防暹罗芽孢杆菌N2发酵培养基及其发酵条件优化[J].吉林农业大学学报,2025,47(2):264-272,9.基金项目
吉林省科技发展计划项目(20180201003NY,20180311001YY),"111基地"建设项目(D17014) （20180201003NY,20180311001YY）
