吉林农业大学学报2025，Vol.47Issue(2)：273-280,8.DOI:10.13327/j.jjlau.2021.1453
常压室温等离子体诱变毛头鬼伞降解玉米秸秆木质素的研究
Atmospheric and Room Temperature Plasma Mutagenized Coprinus comatus for Degradation of Lignin from Corn Straw
摘要
Abstract
Coprinus comatus was mutagenized by atmospheric and room temperature plasma(ARTP),and a mutant library was constructed.A mutant Y9 was screened,which got stronger lignin degrada-tion ability and stable inheritance.The degradation rate for alkali lignin of the mutant was 44.22%,10.71%higher than that of the original strain.The degradation rate of corn straw was 44.52%,with an increase of 15.16%.The Plackett-Burman experiment design was used to determine the three fac-tors that significantly affect degradation of corn straw lignin by Coprinus comatus,inoculation amount,fermentation time,and solid-liquid ratio.Response surface methodology was conducted to optimize lignin degradation capacity of mutants.The optimal fermentation conditions for mutant Y9 were as follows:yeast powder 2 g/L,initial pH of the culture medium 8,temperature 25℃,fermenta-tion time 16 days,solid-liquid ratio 1∶3,and inoculation amount 4%.The lignin degradation rate of corn straw reached 47.32%,6.28%higher than the original strain.This study provides a high quality strain for further application of Coprinus comatus for straw degradation.关键词
毛头鬼伞/木质素/玉米秸秆/响应面法/常压室温等离子体诱变技术Key words
Coprinus comatus/lignin/corn straw/response surface methodology/atmospheric and room temperature plasma mutagenized分类
农业科技引用本文复制引用
苏玉春,李文斌,闫浩然,汪树生,陈光..常压室温等离子体诱变毛头鬼伞降解玉米秸秆木质素的研究[J].吉林农业大学学报,2025,47(2):273-280,8.基金项目
吉林省教育厅项目(JJKH20180688KJ),吉林省大学生创新创业大赛项目(S202110193113) （JJKH20180688KJ）
