吉林农业大学学报2025，Vol.47Issue(2)：281-288,8.DOI:10.13327/j.jjlau.2021.1994
响应面优化乙二胺四乙酸二钠提取荷叶离褶伞子实体多糖工艺条件
Response Surface Optimlization of Ethylenediaminetetraacetic Acid Disodium Salt Extraction Conditions of Polysaccharides from Sporo-phore of Lyophyllum decaste
摘要
Abstract
To enhance the purity of polysaccharides(LDP)extracted from the fruiting bodies of Lyophyllum decastes,a single-factor experiment was conducted as the foundation.The Box-Behnken central composite design principle was applied,with LDP content as the optimization indicator,to op-timize the conditions of three factors:solid-to-liquid ratio,extraction time,and disodium ethylenedi-aminetetraacetate(EDTA-2Na).The results showed that the highest LDP content of 75.47%was achieved when the solid-to-liquid ratio(g:mL)was 1∶22,the extraction time was 3 hours,and the EDTA-2Na addition was 1%.In the response surface optimization experiment,the factors affecting LDP content in descending order were solid-to-liquid ratio,extraction time,and EDTA-2Na.Analysis of monosaccharide composition and relative molecular weight revealed that the retention time of LDP was 10.117 minutes,with a relative molecular weight of 51 770 and a purity of 83%.The monosac-charide composition was primarily glucose and galactose,accompanied by a certain amount of man-nose,fucose,glucuronic acid,ribose,and a small amount of xylose.The findings of this study will provide a reference for further research on polysaccharides from Lyophyllum decastes.关键词
荷叶离褶伞/多糖/EDTA-2Na提取/响应面设计/超声波辅助Key words
Lyophyllum decastes/polysaccharide/EDTA-2Na extraction/response surface de-sign/ultrasound-assisted method分类
农业科技引用本文复制引用
张妍,高敏,戴映迪,苏玲,王琦..响应面优化乙二胺四乙酸二钠提取荷叶离褶伞子实体多糖工艺条件[J].吉林农业大学学报,2025,47(2):281-288,8.基金项目
现代农业产业技术体系建设项目(CARS20-08B),吉林省科技发展计划项目(20220101339JC) （CARS20-08B）
