吉林农业大学学报2025，Vol.47Issue(2)：311-322,12.DOI:10.13327/j.jjlau.2022.1932
不同地力耕作土壤有机碳对外源输入碳的响应及土壤固碳的微生物学机制
Response of Soil Organic Carbon to Exogenous Carbon Input under Different Fertility Levels and Microbiological Mechanism of Soil Car-bon Sequestration
摘要
Abstract
Soil carbon sequestration ability depends not only on soil carbon sequestration capacity but also on soil organic carbon stability.Soil organic carbon is the substrate and metabolite of soil mi-crobial energy metabolism and its enzymatic action.Soil microbes dominate the energy flow and nu-trient cycling of soil ecosystems,and their diversity is critical to soil health.Straw can provide suffi-cient nitrogen and carbon sources for soil microorganisms,promote growth and reproduction of micro-organisms,and affect composition and content of soil organic carbon.Therefore,in order to explore the biological mechanism of soil carbon sequestration under different soil tillage levels,this study used straw returning to the field to carry out organic fertilizer treatments with different straw amounts to black soil with high,medium,and low soil fertility.It further investigated the response of soil or-ganic carbon to the exogenous carbon input under different soil fertility levels and conducted a pre-liminary exploration of the community structure characteristics and metabolic features of soil carbon-sequestering microorganisms.The results showed that the input of external carbon can significantly increase the content of each component of soil humus in different tillage.Compared with the control group,the total humic carbon content and humic acid carbon content of high soil fertility increased by 4.84%and 46.58%,respectively(P<0.05);Compared with the control group,the soil fulvic acid carbon content increased by 13.97 g/kg in the medium soil fertility level;Compared with the control group,the water-soluble carbon content of low soil fertility soil increased by 5.00 g/kg(P<0.05).There is no significant difference in the Alpha diversity index between high soil fertility,medium soil fertility,low soil fertility,and control soil samples,and 2 435,2 407,2 287,and 2 401 OTUs were ob-tained,respectively;Beta diversity analysis found that there were significant differences between dif-ferent soil samples(Stress=0.131 8).The relative abundance of different species changed signifi-cantly after straw returning,and the relative abundance of 28 genera increased significantly.High soil fertility has a unique DLD carbon metabolism pathway,while medium and low soil fertility and control soils have a GAPDH pathway.Therefore,straw returning to the field can increase the content of each component in high,medium,and low soil fertility,resulting in differences in the community structure of carbon-sequestering microbial communities in soils with different soil fertility.关键词
秸秆/腐殖质/耕地地力/土壤微生物/微生物多样性/高通量测序Key words
straw/humus/cultivated land fertility/soil microorganism/microbial diversity/high-throughput sequencing分类
农业科技引用本文复制引用
王舒怡,崔俊涛..不同地力耕作土壤有机碳对外源输入碳的响应及土壤固碳的微生物学机制[J].吉林农业大学学报,2025,47(2):311-322,12.基金项目
吉林省重点研发计划项目(20210204170YY) （20210204170YY）
