江西农业学报2025,Vol.37Issue(4):89-94,111,7.DOI:10.19386/j.cnki.jxnyxb.2025.04.012
不同浓度丁香酚对河川沙塘鳢抗氧化酶活性和组织显微结构的影响
Effects of Different Concentrations of Eugenol on Antioxidant Enzyme Activity and Tissue Microstructure of Odontobutis potamophila
摘要
Abstract
To enhance the survival rate of Odontobutis potamophila during breeding,production,operation and transportation at ambient temperature,this study conducted an anesthesia experiment using eugenol on Odontobutis pota-mophila specimens,and the mass concentrations of eugenol were established at 6,8,10 and 12 mg/L.Following an 8-hour experimental period,alterations in the liver and gill tissues of Odontobutis potamophila were examined.Additionally,the activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-PX),along with variations in malondialdehyde(MDA)content,were assessed in both liver and gill tissues.The results showed that following an 8-hour period,Odontobutis potamophila in each experimental group experienced a full anesthesia and recovery cycle,with a 100%survival rate.Histological examination revealed no significant damage to the liver or gill tissue structures.Following an 8-hour testing period,the activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-PX)in the liver and gill tissues were significantly elevated compared to the control group(P<0.05).In contrast,the malondialdehyde(MDA)content did not differ significantly from the control group.Empirical studies have demonstrated that the application of eugenol during the transportation of Odontobutis potamophila induces an oxidative stress response within the body,without inflicting cellular damage.Consequently,eugenol,when administered at an optimal concentration,can serve as an effective anesthetic for Odonto-butis potamophila during the transportation.关键词
河川沙塘鳢/丁香酚/组织结构/抗氧化酶/麻醉Key words
Odontobutis potamophilus/Eugenol/Tissue structure/Antioxidant enzyme/Anesthesia分类
生物学引用本文复制引用
刘国兴,李杰,周梓涵,郑友,郝忱,丁淑燕,蒋琦辰..不同浓度丁香酚对河川沙塘鳢抗氧化酶活性和组织显微结构的影响[J].江西农业学报,2025,37(4):89-94,111,7.基金项目
江苏现代农业产业技术体系建设项目(JATS[2023]376、JATS[2023]378). (JATS[2023]376、JATS[2023]378)