集美大学学报（自然科学版）2025，Vol.30Issue(2)：103-109,7.DOI:10.19715/j.jmuzr.2025.02.01
基于转录组的黄姑鱼生长相关基因及调控通路研究
Transcriptome-Based Study on Relative Genes and Regulatory Pathways Leading to the Growth of Nibea albiflora
摘要
Abstract
In order to investigate the molecular regulatory mechanisms underlying growth traits in yellow drum(Nibea albiflora),this study selected 110 individuals with faster growth rates(average weight(527.1±83.6)g)and 130 individuals with slower growth rates(average weight(238.4±52.3)g)from the same farming conditions within the same net cage,totaling 240 individuals for study.RNA extraction and transcrip-tome sequencing were performed on muscle tissue from each sample,yielding 499.83 Gb and 576.56 Gb se-quencing data for the faster and slower growth groups,respectively.A total of 149 differentially expressed genes were identified,including 136 upregulated genes and 13 downregulated genes.Functional annotation of differ-entially expressed genes led to the identification of candidate genes related to the muscle growth,such as tnnc1,myh1,tnni2,and myss.GO and KEGG analyses predicted several regulatory pathways associated with muscle growth,with GO term analysis revealing pathways involved in the actin binding,muscle structural com-ponents,and calcium ion binding,while KEGG enrichment analysis identified pathways related to the myocardi-al cell adrenaline and myocardial contraction.These findings provide important insights into the regulatory mechanisms of growth rate in yellow drum,laying a foundation for further studies.关键词
黄姑鱼/转录组/肌肉生长基因/生长速率/调控通路Key words
Nibea albiflora/muscle/transcriptome/growth rate/regulatory pathway分类
水产学引用本文复制引用
李威,刘恬怡,叶坤,王志勇,方铭,姜丹..基于转录组的黄姑鱼生长相关基因及调控通路研究[J].集美大学学报（自然科学版）,2025,30(2):103-109,7.基金项目
国家重点研发计划"重要养殖鱼类基因组选育与单性控制技术"(2018YFD0901201) （2018YFD0901201）
国家自然科学基金项目"基于混池测序技术的黄姑鱼红头病抗病位点超精细定位"(31872560) （31872560）
