集美大学学报（自然科学版）2025，Vol.30Issue(2)：110-120,11.DOI:10.19715/j.jmuzr.2025.02.02
大口黑鲈Dmrt基因家族的鉴定及性别二态性分析
Dentification and Dimorphic Expression of the Dmrt Gene Family in Largemouth Bass(Micropterus salmoides)
摘要
Abstract
In this study,seven Dmrt genes,including Msdmrt1,Msdmrt2a,Msdmrt2b,Msdmrt3,Msdmrt4,Msdmrt5,and Msdmrt6,were identified from the Micropterus salmoides(largemouth bass)genome using bioin-formatics methods.These genes are unevenly distributed across the chromosomes,among which Msdmrt1,Msd-mrt3,and Msdmrt2a are linearly clustered.Phylogenetic analysis revealed that the Msdmrt gene family of large-mouth bass is closely related to that of Larimichthys crocea(large yellow croaker)from the same order,and rela-tively conserved compared to other teleost Dmrt gene families. Combined with transcriptome data,semi-quantitative PCR,and quantitative PCR analyses,theMsdmrt genes showed clear sexually dimorphic expression in the gonads.Msdmrt1,Msdmrt3,Msdmrt4,and Msdmrt6 were specif-ically expressed in the testis,while Msdmrt5 was expressed in both the brain and ovary.These differences in ex-pression may be associated with distinct transcription factor binding sites in the promoter regions of each gene.关键词
大口黑鲈/Dmrt基因家族/性别二态性/染色体分布/系统发育/基因表达模式谱Key words
largemouth bass(Micropterus salmoides)/Dmrt gene family/sexual dimorphism/chromosomal distribution/phylogeny/gene expression profile分类
水产学引用本文复制引用
梁晶婕,姚成杰,陈磊,黄岩,陈天圣..大口黑鲈Dmrt基因家族的鉴定及性别二态性分析[J].集美大学学报（自然科学版）,2025,30(2):110-120,11.基金项目
福建省自然科学基金项目"青鳉dmrt3a在性腺发育中的功能研究"(2022J05149) （2022J05149）
集美大学启动金项目"青鳉双向启动子的筛选、鉴定及应用"(ZQ2022019) （ZQ2022019）
