海洋科学2025，Vol.49Issue(1)：54-67,14.DOI:10.11759/hykx20240417003
转染技术在海洋无脊椎动物中应用现状
Current status of the transfection techniques applied in marine invertebrates
摘要
Abstract
Marine invertebrates harbor immense potential for applications in many fields,such as biomedicine and agriculture.Unraveling and developing their bio-economical value at the genetic level is a major topic for future research.However,genetic manipulation techniques commonly used in model organisms have not yet been widely applied to them;this limitation restricts research on aspects such as gene function and regulation of expression in them.Transfection,as a highly efficient technique for transfection of cells,has become an ideal tool for the genetic manipulation of marine invertebrates.Here,we provide an overview of the different types of transfection methods and compare their advantages and disadvantages.Then,we focus on the progress of transfection technology and summarize the challenges in establishing transfection techniques in marine invertebrates.This study aims to provide a reference for the selection of transfection strategies and to assist in molecular biology research in marine inverte-brates.关键词
转染/海洋无脊椎动物/物理转染/化学转染Key words
transfection/marine invertebrates/physical transfection/chemical transfection分类
海洋学引用本文复制引用
吉中钰,康云思,狄国涛..转染技术在海洋无脊椎动物中应用现状[J].海洋科学,2025,49(1):54-67,14.基金项目
国家自然科学基金(32200415)the National Natural Science Foundation of China,No.32200415 （32200415）
