井冈山大学学报（自然科学版）2025，Vol.46Issue(2)：35-41,7.DOI:10.3969/j.issn.1674-8085.2025.02.005
基于抗原-抗体免疫反应构建荧光传感器检测地龙多肽
A FLUORESCENCE SENSOR BASED ON ANTIGEN-ANTIBODY IMMUNOREACTION WAS CONSTRUCTED FOR THE DETECTION OF EARTHWORM POLYPEPTIDES
摘要
Abstract
Based on the fluorescence characteristics of the complex formed by the binding of polypeptide and its polyclonal antibody,an immunofluorescent sensor for the rapid detection of earthworm polypeptide TP-21 was established for the first time.TP-21 polypeptide and polyclonal antibody had weak fluorescence,but their complex had strong fluorescence.The concentration of TP-21 polypeptide could be inferred by detecting the fluorescence intensity of the complex.The excitation wavelength,buffer system and polyclonal antibody concentration were optimized.Under the optimal conditions,the difference of fluorescence intensity between the complex and polyclonal antibody had a good linear relationship,the linear range was 0.058 59～30.00 μg·mL-1,R2=0.992 7,and the detection limit was 49.22 ng·mL-1.The method is simple,rapid,sensitive and has good selectivity for TP-21 peptide.The content of TP-21 polypeptide in earthworm protein powder was determined.The recovery rates ranged from 85.40％to 105.68％.The results showed that the determination was accurate and reliable.关键词
地龙/荧光免疫分析/多肽检测Key words
earthworm/fluorescent immunoassay/polypeptide detection分类
计算机与自动化引用本文复制引用
倪林君,刘晓凤,刘维清,胡筱,余丽双..基于抗原-抗体免疫反应构建荧光传感器检测地龙多肽[J].井冈山大学学报（自然科学版）,2025,46(2):35-41,7.基金项目
国家自然科学基金项目(82074005) （82074005）
福建省科技厅科技计划项目(2021J01925) （2021J01925）
