华南农业大学学报2025，Vol.46Issue(3)：319-325,7.DOI:10.7671/j.issn.1001-411X.202411013
表达副猪格拉瑟菌GAPDH和OmP26的重组猪霍乱沙门氏菌构建及免疫学评价
Construction and immunological evaluation of recombinant Salmonella choleraesuis expressing GAPDH and OmP26 of Glaesserella parasuis
摘要
Abstract
[Objective]The objective of this study was to construct a recombinant Salmonella choleraesuis strain co-expressing GAPDH and OmP26,two immunogenic antigens derived from Glaesserella parasuis,evaluate its potential as a bivalent vaccine candidate,and provide a novel and efficacious solution for the prevention of both G.parasuis and S.choleraesuis infections in swine populations.[Method]GAPDH and OmP26,which are widely present in various serotypes of G.parasuis,were selected as exogenous antigens.A recombinant strain of G.parasuis-S.choleraesuis,which was capable of expressing both the GAPDH and OmP26,was constructed by using S.choleraesuis C500Δasd deletion strain as vector.The biological characteristic and immune effect of the recombinant strain were then investigated.[Result]Results of PCR and Sanger-sequencing showed that we successfully constructed the recombinant strain C501(pYA-GAPDH-OmP26),which was able to stably harbor GAPDH and OmP26(sizes of 1 020 and 798 bp,respectively).The target fragments were stably amplified from the strain in 100 consecutive passages,and the recombinant strain was consistent with the parent strain C501(pYA3493)in terms of growth curves and biochemical characteristics.The recombinant strain C501(pYA-GAPDH-OmP26)showed 62.5%and 50.0%protection rates against S.choleraesuis C78-1 and G.parasuis type 5 strong strain SH0165,respectively,while C501(pYA3493)showed 50.0%protection rate against S.choleraesuis C78-1 and no protection effect against G.parasuis type 5 strong strain SH0165.[Conclusion]The recombinant S.choleraesuis C501(pYA-GAPDH-OmP26)can stably carry heterologous genes.Compared with the parent strain,the recombinant strain has similar biological and good expression characteristics,can induce the organism's combined immune response against G.parasuis and S.choleraesuis.The study lays the foundation for the development of the bivalent genetically engineered vaccine for G.parasuis and S.choleraesuis.关键词
猪/副猪格拉瑟菌/猪霍乱沙门氏菌/C500Δasd缺失株/GAPDH/OmP26Key words
Pig/Glaesserella parasuis/Salmonella choleraesuis/C500Δasd deletion strain/GAPDH/OmP26分类
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陈丽璇,符颖,陈政权,李莉莉,张伟孝,张建民..表达副猪格拉瑟菌GAPDH和OmP26的重组猪霍乱沙门氏菌构建及免疫学评价[J].华南农业大学学报,2025,46(3):319-325,7.基金项目
"十四五"国家重点研发计划(2022YFD1800901) （2022YFD1800901）
