华南农业大学学报2025，Vol.46Issue(3)：351-357,7.DOI:10.7671/j.issn.1001-411X.202408023
tva受体基因起始密码子突变对清远麻鸡感染K亚群禽白血病病毒的影响
Effect of initiation codon mutation within tva gene on Qingyuan partridge chicken resistance to infection by avian leukemia virus subgroup K
摘要
Abstract
[Objective]To investigate the effect of initiation codon mutation within tva receptor gene(tva c.3G＞A)on Qingyuan partridge chicken resistance to infection by avian leukosis virus subgroup K(ALV-K).[Method]The tva c.3G＞A mutation site within Qingyuan partridge chicken lines was genotyped by using direct sequencing method.The infection of RCASBP(K)-EGFP fluorescent reporter virus and ALV-K GD1601 virus to chicken embryo fibroblasts(CEFs)with different genotypes of tva c.3G＞A mutation site were detected by flow cytometry and RT-qPCR,respectively.The infection of ALV-K GD1601 virus to Qingyuan partridge chicks with different genotypes of tva c.3G＞A mutation site were detected by RT-qPCR.[Result]The genotyping results of tva c.3G＞A mutation site showed that the heterozygous mutant genotype tva c.3G＞A and the homozygous mutant genotype tva c.3A/A existed in Qingyuan partridge chicken lines K and M,with the frequencies of 0.183 and 0.133 for tva c.3G/A,0.116 and 0.033 for tva c.3A/A,respectively.The results of flow cytometry and RT-qPCR showed that the tva c.3G＞A mutation site wild-type tva c.3G/G CEFs were susceptible to infection of both RCASBP(K)-EGFP and ALV-K GD1601 virus,while the homozygous mutant genetype tva c.3A/A CEFs were effectively resistant to infection of RCASBP(K)-EGFP and ALV-K GD1601 viruses,demonstating that tva c.3G＞A mutation caused Qingyuan partridge chicken resistance to infection of ALV-K in vitro.The results of ALV-K challenge test indicated that tva c.3G＞A mutation led to Qingyuan partridge chicks resistance to infection of ALV-K in vivo.[Conclusion]The tva c.3G＞A mutation confers Qingyuan partridge chicken resistance to infection of ALV-K both in vitro and in vivo,indicating that it can be used as a genetic marker for ALV-K resistance selection in Qingyuan partridge chicken.关键词
清远麻鸡/K亚群禽白血病病毒/tva受体基因/突变/遗传抗性Key words
Qingyuan partridge chicken/Avian leukosis virus subgroup K/tva receptor gene/Mutation/Genetic resistance分类
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徐慧娟,邝智祥,左珂菁,吴承洋,郑泓昊,谢青梅,陈伟国..tva受体基因起始密码子突变对清远麻鸡感染K亚群禽白血病病毒的影响[J].华南农业大学学报,2025,46(3):351-357,7.基金项目
国家重点研发计划子课题(2021YFD1300105) （2021YFD1300105）
清远市科技计划(2021DZX019) （2021DZX019）
