河南农业科学2025，Vol.54Issue(3)：1-7,7.DOI:10.15933/j.cnki.1004-3268.2025.03.001
水稻蔗糖转运蛋白OsSUT4基因的克隆与功能分析
Cloning and Function Analysis of Rice Sucrose Transporter OsSUT4 Gene
摘要
Abstract
The rice sucrose transporter gene OsSUT4 was cloned,and sucrose transport capability of OsSUT4 was verified by yeast heterologous expression system.The gene was overexpressed in rice,the phenotype of transgenic plants was observed,and the salt tolerance and drought resistance was identified,so as to explore the role of the OsSUT4 gene in stress resistance in rice and provide new gene resources and theoretical foundations for rice genetic improvement.The results showed that OsSUT4 gene was primarily expressed in the flag leaves at jointing stage;The sucrose transport capability of OsSUT4 was confirmed for the first time using the yeast heterologous expression system;Overexpression of OsSUT4 gene did not significantly alter the grain morphology or the thousand-grain weight,but significantly increased plant height and survival rates under salt and drought stresses,thereby enhancing the salt tolerance and drought resistance of rice.关键词
水稻/蔗糖转运蛋白/过表达/株高/耐盐性/抗旱性Key words
Rice/Sucrose transporter/Overexpression/Plant height/Salt tolerance/Drought resistance分类
农业科技引用本文复制引用
蔡昱萌,李军玲,汪颖,何飞,刘燕清,刘静妍,郭彦丽,孙玥,苏京平,王胜军..水稻蔗糖转运蛋白OsSUT4基因的克隆与功能分析[J].河南农业科学,2025,54(3):1-7,7.基金项目
天津市农业科学院青年科技创新项目(2021013,2021004,2021007) （2021013,2021004,2021007）
