江西农业大学学报2025，Vol.47Issue(2)：302-313,12.DOI:10.3724/aauj.2025027
苦荞RILs群体千粒质量和粒形的遗传特性分析
Analysis of genetic characteristics of thousand-grain weight and grain shape in RILs population of Tartary buckwheat
摘要
Abstract
[Objective]Thousand grain weight is one of the three major components of yield traits in Tartary buckwheat,which is determined by grain size(grain length,grain width and grain thickness)and grain plumpness.Studying genetic variation and molecular mechanism of grain weight and grain size is of great significance for Tartary buckwheat breeding.[Method]In the study,grain length(GL),grain width(GW),length to width ratio(L/W)and 1000-grain weight(TGW)of'Jinqiaomai 2×Pinku 1'recombinant inbred lines(JP-RILs)population were subjected to variation analysis,mixed major genes+polygene genetic model analysis,correlation analysis and cluster analysis under two environments.[Result]A continuous distribution and transgressive segregative distribution was observed for the four tested traits in the JP-RILs population under two environments.The coefficient of variation was the largest for L/W(15.30%,15.70%),followed by GL(11.27%,11.03%),and was the smallest for GW(5.46%,5.94%).The optimal genetic model of GW was both MX1-A-AI under two environments,which was controlled by one additive epistatic major gene,with the heritability of the major gene of 69.84%and 62.68%,and that of the polygene of 23.90%and 34.24%.The optimal genetic model of L/W was both 4MG-AI under two environments,which was controlled by four additive major genes,with the heritability of the major gene of 98.07%and 98.26%.The optimal genetic model of GL was 4MG-AI under E1 and 1MG-A under E2 environment,which was controlled by four additive major genes and one additive major gene with the heritability of the major gene of 97.98%and 88.39%,respectively.The optimal genetic model of TGW was 2MG-DE under E1 and 4MG-EEEA under E2 environment,which was controlled by two duplicate major genes and four partly additive major genes with the heritability of the major gene of 36.61%and 70.43%,respectively.Lines with high TGW and long-tapered grains or round-shaped grains and lines with low TGW and round-shaped grains were screened by cluster analysis,which can be considered as breeding materials for new varieties and also provide good materials for genetic research.[Conclusion]Grain size and TGW were controlled by major genes,and the epistasis effects of major genes was highly predominant,which laid a basic foundation for QTL mapping and genetic improvement for grain size and TGW in Tartary buckwheat.关键词
苦荞/重组自交系/粒形/主基因+多基因遗传模型/相关性分析/聚类分析Key words
Tartary buckwheat/RILs population/grain size/major gene plus polygene model/correlation analysis/cluster analysis分类
农业科学引用本文复制引用
石茂竹,黎瑞源,刘迪,陈庆富,王莉花,石桃雄..苦荞RILs群体千粒质量和粒形的遗传特性分析[J].江西农业大学学报,2025,47(2):302-313,12.基金项目
国家自然科学基金项目(32260489)、国家现代农业产业体系荞麦育种岗位科学家专项资金(CARS-07-A5)和云南省重大科技专项与重点研发计划项目(202202AE090020)Project supported by the National Natural Science Foundation of China(32260489),National Modern Agricultur-al Industry System Buckwheat Breeding Post Scientist Special Fund(CARS-07-A5)and Major Science and Tech-nology Project of Yunnan Province(202202AE090020) （32260489）
