江西农业大学学报2025，Vol.47Issue(2)：314-324,11.DOI:10.3724/aauj.2025028
不同抗性青稞品种响应啶磺草胺胁迫的代谢组差异分析
Analysis of differences in metabolism of pyrimoxsulam resistance in different resistant barley varieties
摘要
Abstract
[Objective]This study aims to explore the important metabolites that may be involved in the metabolism of pyrimoxsulam in hulless barley,as well as clarify the metabolism mechanism of pyrimoxsulam in hulless barley,thus providing a theoretical basis for the scientific use of pyrimoxsulam and the selection and breeding of herbicide-tolerant hulless barley varieties.[Method]The leaves of a tolerant hulless barley variety(Qing 0306)and a sensitive variety(Qing 0160)treated with pyrimoxsulam for 0 d,1 d and 6 d were analyzed by liquid chromatography-mass spectrometry(LC-MS).[Result]With VIP>1.0 and P<0.05 as the threshold,598 differential metabolites were screened out,which were mainly flavonoids,terpenoids compounds,alcohols,phthalides and other substances;KEGG metabolic pathway was enriched and analyzed,which was mainly involved in metabolism of metabolites 2-oxocarboxylic acid metabolism,amino acid biosynthesis as well as flavonoid biosynthesis,among which the biosynthesis of flavonoids is the most significant.[Conclusion]Using metabolomics technology,different varieties of hulless barley leaves were analyzed by metabolomics in different periods(0,1,6 d)after pyrimoxsulam stress,the metabolite changes in sensitive and resistant hulless barley varieties after pyrimoxsulam treatment were clarified.This study provides a basis for the subsequent genetic improvement of hulless barley and the scientific application of pesticides.关键词
青稞/啶磺草胺/代谢组学/差异代谢物/胁迫/耐药机制Key words
hulless barley/pyrimoxsulam/metabolomics/differential metabolites/stress/mechanisms of resistance分类
农业科学引用本文复制引用
秦云卓,翁华..不同抗性青稞品种响应啶磺草胺胁迫的代谢组差异分析[J].江西农业大学学报,2025,47(2):314-324,11.基金项目
青海省重点研发与转化项目(2023-NK-154)Project supported by Qinghai Province Key Research and Development and Transformation Program(2023-NK-154) （2023-NK-154）
