江西农业大学学报2025，Vol.47Issue(2)：349-359,11.DOI:10.3724/aauj.2025031
原生质体融合获得羊肚菌黑色栽培种与黄色非栽培种间杂交高产菌株
Protoplast fusion to obtain high-yield strains from interspecific hybridization between black cultivated species and yellow uncultured species of Morchella
摘要
Abstract
[Objective]At present,the main issues that restricting the development of Morchella industry in Jiangxi Province are low yield,difficulty in replicating high yields,and limited availability of high-quality strains,and the key to addressing these challenges lies in the improvement of strains.The purpose of this study is to breed a new strain of Morchella with high yield,which is suitable for local cultivation in Jiangxi.[Method]According to the theory of interaction between heredity and environment,targeted selection was made of the wild yellow Morchella strain JFRL01-161 collected locally in Jiangxi,along with the commercially cultivated Morchella importuna strain JFRL01-107 and Morchella sextelata strain JFRL01-217 from Yingtan region of Jiangxi Province,which were used as original strains,and protoplasts were prepared by dissolving the cell wall with a complex enzyme composed of 20 g/L lywallzyme and 10 g/L snail enzyme,and then two kinds of inactivated protoplasts were obtained by high temperature and ultraviolet double inactivation.The obtained fusants were tested by antagonism,cultivar ratio and Inter-Simple Sequence Repeat to determine the provenance relationship.[Result]Sixteen fusion strains were obtained by protoplast fusion,namely A11,A37,A39,B7,B12,B17,B18,B25,B26,B29,C1,C2,C5,C6,C11 and C12.Among them,strains A11,A37,A39 and B18 successfully fruited and produced yields,the yield of the fusion strain A11 reached 351.90 g/m2,which was significantly higher than that of the parent JFRL01-107(264.51 g/m2).The annual repeated cultivation verification test confirmed that the yield of A11 could reach 527.8 g/m2,and that of the parent JFRL01-107 was 262.4 g/m2.The phylogenetic tree(UPGMA)of unweighted arithmetic mean clustering system showed that A11 was in different branches from its parents JFRL01-161(similarity coefficient 0.24)and JFRL01-107(similarity coefficient level 0.696),which proved that A11 was a new strain different from its parents.[Conclusion]The yield of fused strain A11 was significantly higher than that of its parents.Repeated cultivation experiments confirmed that its germplasm was stable,and ISSR confirmed that it was different from its parents.Therefore,fused strain A11 can be used as a new strain of high-yield Morchella adapted to local cultivation in Jiangxi.关键词
羊肚菌/高产羊肚菌新菌株A11/紫外和热双灭活/原生质体融合/区间简单重复序列标记分析Key words
Morchella/new strain of Morchella esculenta with high yield A11/UV and heat double inactivation/protoplast fusion/marker analysis of interval simple repeat sequence分类
农业科学引用本文复制引用
廖武俊,钟啟萍,李荣光,霍达,霍光华..原生质体融合获得羊肚菌黑色栽培种与黄色非栽培种间杂交高产菌株[J].江西农业大学学报,2025,47(2):349-359,11.基金项目
江西省重点研发计划项目(20203BBF63036)Project supported by the Key Research and Development Plan of Jiangxi Province(20203BBF63036) （20203BBF63036）
