江西农业大学学报2025，Vol.47Issue(2)：360-372,13.DOI:10.3724/aauj.2025032
叶面喷施CaCl2/SA/EBR对南酸枣幼苗抗寒性的影响
Effects of foliar application of CaCl2/SA/EBR on cold resistance of Choerospondias axillaris seedlings
摘要
Abstract
[Objective]The effects of spraying calcium chloride(CaCl2),salicylic acid(SA)and 2,4-epibrassinolide(EBR)on the cold resistance of Choerospondias axillaris seedlings are investigated,and the treatment and concentration of enhancing the cold resistance of seedlings are screened out to provide a basis for enhancing the cold resistance of C.axillaris seedlings to low temperature in winter or late spring cold snaps.[Method]Using one-year-old potted seedlings of C.axillaris derived from seeds as the experimental materials,a randomized block experimental design was adopted.From November 10th to 20th,the seedlings of C.axillaris in three experimental plot groups were respectively subjected to foliar spraying of CaCl₂(C1,C2,C3),SA(S1,S2,S3)and EBR(B1,B2,B3)six times(once every two days),and then the seedling branches of each spraying treatment were treated with low temperature stress at 4℃for 12 h.The anatomical structure of the leaves after stress treatment was observed by paraffin section method,and the changes of physiological indexes of the branches were measured.[Result]Compared with spraying water(CK),the cell density of palisade tissue in leaves of C.axillaris increased after spraying CaCl2,SA and EBR.Among them,the upper epidermal thickness was the largest in the treatment of 10 mmol/L SA.The thickness of the lower epidermis treated with 1 mmol/L CaCl2 was the largest,and the palisade tissue treated with 0.1 mg/L EBR was thicker.Under the treatment of 10 mmol/L CaCl2,the thickness of sponge tissue,leaf thickness and leaf porosity increased,and the ratio of palisade tissue to spongy tissue and leaf tightness decreased.After spraying 20 mmol/L SA,the relative conductivity content in the stem of C.axillaris was significantly lower than that of CK,which was 12.07%.The content of malondialdehyde after 0.1 mg/L EBR treatment was lower than that of other treatments and CK,which was only 0.039 μmol/g.In addition to spraying 0.01 mg/L EBR and 0.1 mg/L EBR,the soluble sugar content in the stem of C.axillaris was slightly higher than that of the control,and the soluble sugar under other treatments was reduced to varying degrees compared with the control.Compared with CK,after spraying CaCl2,SA and EBR,the contents of soluble protein and proline(except 1 mg/L EBR)and the activities of SOD and POD(except 10 mmol/L CaCl2)in the stems of C.axillaris were increased by varying degrees.Through the principal component analysis of leaf anatomical structure index and physiological index,the treatment effect of three different exogenous substances from strong to weak was 10 mmol/L SA,1 mmol/L CaCl2,20 mmol/L CaCl2,1 mmol/L SA,10 mmol/L CaCl2,20 mmol/L SA,0.1mg/L EBR,0.01 mg/L EBR,1 mg/L EBR,CK.[Conclusion]The treatment of three different exogenous substances is beneficial to enhance the cold resistance of C.axillaris seedlings.The effect of SA treatment is better than that of CaCl2 and EBR,and the cold resistance of C.axillaris treated with 10 mmol/L SA is the best.关键词
南酸枣幼苗/外源物质/叶片解剖结构/生理特征/抗寒性Key words
Choerospondias axillaris seedlings/exogenous substances/leaf anatomical structure/physiological characteristics/cold resistance分类
林学引用本文复制引用
朱秋敏,杨如梦,汪加魏,吴南生,高岩,曹嘉晟,丁菲,孙荣喜,张露..叶面喷施CaCl2/SA/EBR对南酸枣幼苗抗寒性的影响[J].江西农业大学学报,2025,47(2):360-372,13.基金项目
国家自然科学基金项目(32160387)和江西省重点研发计划项目(20171BBF60011)Project supported by the National Natural Science Foundation of China(32160387)and Key R&D Program of Ji-angxi Province(20171BBF60011) （32160387）
