菌物研究2025，Vol.23Issue(2)：151-161,11.DOI:10.13341/j.jfr.2024.1794
基于非靶向代谢组学探究灵芝连作对孢子粉代谢差异的影响
Analysis of Metabolic Differences in Ganoderma lingzhi Spore Powder under Continuous Cropping Using Untargeted Metabolomics
摘要
Abstract
With the method of untargeted metabolomics,this study aims to investigate the effects of continuous cropping of Ganoderma lingzhi over two years on its metabolic passways within its spore powder.By growing G.lingzhi on hardwood logs in a greenhouse,we compared the biological indicators and metabolite profiles of harvested spore powder between the first year(SP1)and the second year(SP2).Results show the fruiting body yield,spore powder yield,total sugar content,and triterpenoid content of SP1 were all higher than those of SP2,with significant differences in fruiting body yield and total sugar content(P<0.05),indicating that the spore powder produced in the first year had a clear quality advantage.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)revealed the significant differences in metabolite compositions between the two spore powder samples,with a cumulative contribution rate of 0.74 from the PCA model and a good prediction ability(Q2=0.31,R2Y=0.95)from the OPLS-DA model.Differential analysis indicated that contents of 33 metabolites were significantly higher while 21 metabolites were significantly lower in SP1 than those in SP2(P<0.05).Major differential metabolites in SP1 included oleic acid,ganoderic acid and schisandrin,involving triterpenes,glycerophospholipids,and carboxylic acids and their derivatives whereas the major differential metabolites in SP2 were curcumin,n-decanol and 1-nonanol involving carboxylic acids and their derivatives,phenolics and organic oxidants.Enrichment analysis of KEGG pathway revealed that the continuous cropping of two years made significant influences on multiple metabolic pathways,such as amino acid biosynthesis and purine metabolism.Hierarchical analysis of metabolic pathways and the enrichment of key metabolites(L-threonine and D-aspartic acid)suggest that metabolic regulation is the primary driving force behind the differences in spore powder.Significant differences detected from the pathways relating to ABC transporters,2-oxo acid metabolism and amino acid biosynthesis also suggest their crucial role in the metabolic regulation of Ganoderma spore powder.The metabolic network reprogramming induced by continuous cultivation not only reflects G.lingzhi's adaptive mechanisms to environmental stress,but also provides theoretical insight into improving spore powder quality and cultivation efficiency.Moreover,continuous cropping significantly alters the composition of spore powder metabolites,offering valuable data support for the cultivation and metabolic regulation of G.lingzhi.关键词
灵芝孢子粉/代谢组学/灵芝连作/差异代谢物/KEGG通路分析Key words
Ganoderma lingzhi spore powder/metabolomics/continuous cropping/differential me-tabolites/KEGG pathway analysis分类
生物学引用本文复制引用
纪伟,苏文英,刘晓梅,王一璞,王宝祥,任立凯,陈克龙..基于非靶向代谢组学探究灵芝连作对孢子粉代谢差异的影响[J].菌物研究,2025,23(2):151-161,11.基金项目
江苏省重点研发计划项目(BE2023376),连云港市财政专项(QNJJ2410),连云港市科协软课题(重点资助)研究项目(Lkxzd2405) （BE2023376）
