菌物研究2025，Vol.23Issue(2)：162-174,13.DOI:10.13341/j.jfr.2024.1797
皱皮粗柄鸡枞菌子实体发育的关联及调控物质分析
Analysis of Associative and Regulatory Substances on Fruiting Body Development of Termitomyces robustus
摘要
Abstract
Termitomyces mycelia could not twist to develop into a fruiting body on the culture media.In order to reveal those substances potentially relating to development of fruiting bodies,metabolites isolated from the fruiting bodies and purely cultured mycelia of Termitomyces robustus were analyzed by using liquid chromatography-mass spectrometry(LC-MS).A total of 1 424 and 1 457 substances were detected within the above two tissues of fungi respectively,of which 212 were specific to fruiting bodies while 245 were specific to mycelia.There existed 543 kinds of significantly differential substances between the fruiting body and the mycelium,including 377 up-regulated and 166 down-regulated substances.Cytoscape software was used to demonstrate the network associations between the above tissue-specific substances that revealed a presence of 58 commonly differential substances.KEGG enrichment analysis indicated an occurrence of upstream and downstream regulation association for 23 tissue-specific substances(14 from mycelia and nine from fruiting bodies)and 38 differential substances(27 up-regulated and 11 down-regulated)respectively under four and nine common pathways.The above findings,thereafter,enable us to suggest that these substances,especially fruitingbody-specific substances and up-regulated substances,have certain regulatory effects on the development of fruiting body,in which 126 substances(65 showing the relative content≥2 and 61 enriched under the pathways)revealed significant or close relationships between mycelia and fruiting bodies.Our results thus provide a theoretical basis for further analysis of signal molecules and screening of facilitatory metabolites in relation to the development of Termitomyces fruiting bodies,large-scale artificial cultures of Termitomyces and drug development of the anti-tumor and others as well.关键词
皱皮粗柄鸡枞菌/子实体/菌丝体/关联物质/调控物质Key words
Termitomyces robustus/fruiting bodies/mycelia/associative substances/regulatory substances分类
生物学引用本文复制引用
刘夏,王成凤,张曦予,张实润,董杰,刘佳艳,李宗菊..皱皮粗柄鸡枞菌子实体发育的关联及调控物质分析[J].菌物研究,2025,23(2):162-174,13.基金项目
国家自然科学基金项目(31560575),云南省自然科学基金项目(2014FA020) （31560575）
