林产化学与工业2025，Vol.45Issue(2)：1-9,9.DOI:10.20195/j.issn.0253-2417.2024112
全二维气相色谱-质谱顶空分析总状土木香精油成分
Rapid Analysis of the Components of Essential Oil of Inula racemosa Hook.f.in the Headspace of the Comprehensive Two-dimensional Temperament
摘要
Abstract
The essential oil of Inula racemosa Hook.f.from Sichuan Province was extracted by steam distillation.A comprehensive two-dimensional gas chromatography-mass spectrometry(GC × GC-MS)analysis method of essential oil of I.racemosa was established,and the GC × GC-MS analysis of volatile components was carried out by headspace sampling method.Based on single-factor experiments,the optimized process conditions of essential oil extraction were as follows:particle size of 0.850 mm,soaking time of 6 h,extraction time of 10 h,liquid-to-material ratio of 10∶1(mL∶g),with an essential oil yield of 1.02％under these conditions.GC × GC-MS was used to analyze the volatile components of I.racemosa under two different extraction methods.A total of 30 components were identified by steam distillation,while 82 components were identified by headspace sampling,with 8 common components,mainly sesquiterpenes.The volatile components and relative contents obtained from I.racemosa showed some differences.The main essential oil components and GC contents identified by steam distillation were 29.65％of alantolactone,at 28.11％of β-elemene,and 12.88％of β-selinene,respectively.In contrast,the main volatile components and relative contents identified by headspace sampling were at 46.34％of alantolactone and 41.17％of isoalantolactone,respectively.The optimized essential oil extraction process and headspace sampling method could be combined to obtain a more comprehensive volatile substances of I.racemosa.Utilizing GC × GC-MS technology for component analysis could serve as a rapid identification method for evaluating the quality of I.racemosa essential oil.关键词
总状土木香/精油/全二维气相色谱-质谱技术/化学成分Key words
Inula racemosa Hook.f/essential oil/comprehensive two-dimensional gas chromatography-mass spectrometry technology/chemical components分类
化学化工引用本文复制引用
严志宏,许金娣,张臣,顿珠次仁,张宁方,罗珍..全二维气相色谱-质谱顶空分析总状土木香精油成分[J].林产化学与工业,2025,45(2):1-9,9.基金项目
国家自然科学基金资助项目(82160827) （82160827）
2021年西藏藏医药大学中药学(藏药)博士点培育科研支撑计划项目(BSDPY-21-08) （藏药）
2024藏药博士点建设学科建设项目(BSDJS-XKJS-24-04) （BSDJS-XKJS-24-04）
