林产化学与工业2025，Vol.45Issue(2)：19-25,7.DOI:10.20195/j.issn.0253-2417.2024120
数字图像比色法测定肉桂油中醛类化合物的研究
Detection of Aldehydes in Cinnamon Oil by Digital Image Colorimetry Method
摘要
Abstract
Based on the principle that aldehydes could react with 2,4-dinitrophenylhydrazine(DNPH)to produce red chromogenic substances,the images of colored parts were collected and the three-color red,green and blue(RGB)mode was used to obtain data.A digital image colorimetry method was established to detect aldehydes and applied to cinnamon oil samples,and the conditions of detection method were optimized and the methodology was verified.The results showed that for 20 μL samples to be tested,1 mL DNPH(5 mmol/L),10 μL hydrochloric acid and 1.5 mL potassium hydroxide(60 g/L)were added to complete the color reaction,and the cinnamaldehyde measurement method could be established using the blue/green(B/G)value of the sample color as an indicator.The correlation coefficient of the standard curve of the method was 0.998 6,the limit of quantitation was 1 mg/L,the detection limit was 0.5 mg/L,the matrix interference was 6.5％,the intraday and daytime precision were 0.39％and 0.52％,respectively,the accuracy was 102.5％-107.5％,and the relative standard deviation(RSD)was less than 6.5％.关键词
数字图像比色法/2,4-二硝基苯肼/肉桂油/醛类化合物Key words
digital image colorimetry/2,4-dinitrophenylhydrazine/cinnamon oil/aldehydes分类
化学工程引用本文复制引用
程贤,李胜男,李盼盼,陈玉湘,李鹏飞,毕良武..数字图像比色法测定肉桂油中醛类化合物的研究[J].林产化学与工业,2025,45(2):19-25,7.基金项目
国家自然科学基金青年项目(32201512) （32201512）
广西林产化学与工程重点实验室开放基金课题(GXFK2407) （GXFK2407）
