国际眼科杂志2025，Vol.25Issue(5)：689-697,9.DOI:10.3980/j.issn.1672-5123.2025.5.01
小动物视觉电生理数据采集操作规范指南(2025)
Operation guide for the visual electrophysiology data collection in small animals(2025)
摘要
Abstract
Visual electrophysiology measurements have become a routine method of functional examination in ophthalmology.Small animal visual electrophysiology is an important tool for exploring the functionality of the visual system in small animals,finding widespread applications in neuroscience and drug research and development.This guide aims to offer a standardized operational guide for the operation of visual electrophysiological norms in small animals to ensure the accuracy and repeatability of the test.The study emphasizes different types of visual electrophysiological tests,such as electroretinogram(ERG)and visual evoked potential(VEP),evoked in small animals,and their application in different disease models.Detailed descriptions are provided regarding the selection and preparation of experimental animals,including the requirements of animal species,anesthesia methods and test environment.In terms of operational procedures,this guide highlights the correct electrode placement,the selection of stimulus parameters,and the key steps for signal acquisition and processing.According to different animal models,the corresponding operation suggestions were provided,and the troubleshooting methods of common problems were introduced.Beyond fundamental operations,this guide also focuses on the interpretation and reporting of test results.It explains various types of electrophysiological waveforms.In summary,this operational specification for small animal visual electrophysiology provides a comprehensive and detailed framework for researchers to ensure the standardization and reliability of tests.By following these guidelines,researchers can effectively utilize small animal visual electrophysiology techniques to gain insight into the function and abnormalities of the visual system.关键词
小动物视觉电生理/视觉电生理/神经科学/视网膜电反应图/视觉诱发电位操作指南Key words
small animal visual electrophysiology/visual electrophysiology/neuroscience/electroretinogram/visual evoked potential operation guide引用本文复制引用
邵毅,李世迎,颜标,《小动物视觉电生理数据采集操作规范指南2025》专家组,中国医药教育协会眼科影像与智能医疗分会及智能医学专委会,国际转化医学会眼科专业委员会,国际智能医学会眼科专业委员会,中国眼科影像研究专家组..小动物视觉电生理数据采集操作规范指南(2025)[J].国际眼科杂志,2025,25(5):689-697,9.基金项目
国家自然科学基金资助项目(No.82160195,82460203) （No.82160195,82460203）
江西省双千计划科技创新高端领军人才项目(No.jxsq2023201036) （No.jxsq2023201036）
江西省重大(重点)研发专项计划(No.20223BBH80014) National Natural Science Foundation of China(No.82160195,82460203) （重点）
Jiangxi Province Double Thousand Plan Science and Technology Innovation High-end Leading Talents Project(No.jxsq2023201036) （No.jxsq2023201036）
Major(Key)Research and Development Project of Jiangxi Province(No.20223BBH80014) （Key）
