国际眼科杂志2025，Vol.25Issue(5)：706-713,8.DOI:10.3980/j.issn.1672-5123.2025.5.03
基于荧光成像技术验证视网膜母细胞瘤小鼠模型
Validation of retinoblastoma mouse model based on fluorescence imaging technology
摘要
Abstract
AIM:To provide references for the non-clinical evaluation of therapeutic targets or drugs for retinoblastoma,fluorescently labeled Y79 cells are injected into the vitreous body of BALB/c-nu mice to establish a retinoblastoma model,and the Melphalan treatment group is used as a positive control,which is verified by fluorescence imaging technology. METHODS:BALB/c-nu mice were intravitreous injected with GFP transfected Y79 cells(1.0× 107 cell/mL,3 μL)to establish the model.On the 27th day,the mice were randomly divided into model control group and different doses of Melphalan groups(1,3,10 μg/eye groups)according to the fluorescence value of in vivo imaging,with vitreous body single administrated and ocular symptoms observed daily.Slit-lamp examination was performed at 12,20,29,35,42,48,55,76,and 83 d after modeling.In vivo imaging was performed on 12,20,27,41,48,55,62,69,76,and 83 d.At the last treatment,the eyeball,brain and cerebellum tissues were removed for histopathological examination. RESULTS:From the sixth day of modeling,cloud-like substances could be seen in the eyes of the animals,and the cloud-like substances occupied the whole eyeball of the mice in the model control group at the later stage,accompanied by irregular growth of blood vessels.After 27 days of modeling,the fluorescence value was detected in all the animals,and the fluorescence value continued to increase with the extension of modeling time.The fluorescence value of the tumor reached the peak after 69-83 days of modeling.Histological examination showed severe proliferation of intraocular tumor cells in the model control group,and tumor cells were observed in the brain of 1 model animal.In the 10 μg/eye Melphalan group,the fluorescence value was significantly decreased at 17 d after administration.The fluorescence value of the 3 μg/eye Melphalan group was significantly inhibited at 59 d after administration.No tumor cells were found in the brain tissue of animals in all Melphalan groups. CONCLUSION:After vitreous injection of Y79/pCDH-LUC-copGFP cells in BALB/c-nu mice,significant ocular lesions and proliferation of tumor cells were observed in the eyes.Meanwhile,Melphalan intervention significantly inhibited tumor cells in a dose-dependent manner,indicating that the mouse model of retinoblastoma was successfully constructed.关键词
BALB/c-nu小鼠/视网膜母细胞瘤/Y79细胞/活体成像/荧光成像技术Key words
BALB/c-nu mice/retinoblastoma/Y79 cells/in vivo imaging/fluorescence imaging technology引用本文复制引用
代彩玲,杨威,王俐梅,戴锦龙,温玉莹,郭健敏..基于荧光成像技术验证视网膜母细胞瘤小鼠模型[J].国际眼科杂志,2025,25(5):706-713,8.基金项目
广东省海洋经济发展(海洋六大产业)专项资金项目{No.GDNRC[2024]25} Guangdong Province Marine Economy Development(Six Major Marine Industries)Special Funds Project{No.GDNRC[2024]25} （海洋六大产业）
