林业科学研究2025，Vol.38Issue(2)：34-47,14.DOI:10.12403/j.1001-1498.20240230
中国沙棘和蒙古沙棘不同区室细菌微生物组特征与溯源分析
Bacterial Microbiome Characteristics and Traceability Analysis of Different Compartments in Hippophae rhamnoides subsp.sinensis and H.rhamnoides subsp.mongonica
摘要
Abstract
[Objective]Sea buckthorn(Hippophae rhamnoides L.)is a typical actinorhizal plant and a main pioneer afforestation species in the Three-North Region.However,little is known about the overall microbi-ome community structure and nodule nitrogen fixation symbiosis pattern of sea buckthorn.This study sys-tematically analyzed the microbiome characteristics of sea buckthorn by analyzing the effects of different species and compartment niches on the microbiome structure and composition of sea buckthorn.[Meth-od]We employed 16S rRNA gene amplicon sequencing method to study the diversity and composition of microbial communities in the compartment niches of H.rhamnoides subsp.sinensis and H.rhamnoides subsp.mongonica,which have the largest cultivated area in the Three-North Region.[Result]It was found that the diversity and composition structure of microbial communities of H.rhamnoides subsp.sinensis and H.rhamnoides subsp.mongonica were mainly influenced by the compartment niche.The alpha diversity of bacterial communities in the soil compartments(Shannon index ranged from 8.50 to 8.96)was signific-antly higher than that of plant endosphere compartment niches(Shannon index ranged from 4.79 to 8.32),with the nodule endosphere compartment showing the lowest alpha diversity(Shannon index ranged from 4.79 to 5.69).Significant differences were observed in the composition of microbial communities across dif-ferent compartments.Pseudoxanthomonas and Rhizobium were mainly enriched in the root endosphere microbial communities,while Massilia was mainly enriched in the stem endosphere and leaf endosphere compartments.Frankia was dominant in the nodule endosphere compartment and was the sole biomarker for this compartment in both sea buckthorn species.Source tracking analyses indicated that the microbial communities in the root and nodule endosphere compartments were mainly derived from the soils and un-derwent gradual enrichment and filtering.However,the main microbial sources in the stem and leaf endo-sphere compartments was not soil-derived.[Conclusion]Our findings indicate that compartment niches[lay a critical role in affecting the composition structure and symbiosis pattern of sea buckthorns microbial community.Notably,Frankia,a unique symbiont,was dominant in the nodule endosphere,underscoring a specific sea buckthorn-Frankia symbiosis.These results provide an important theoretical basis for a deep-er understanding of the adaptation mechanism of sea buckthorn to the environment.关键词
蒙古沙棘/中国沙棘/区室生态位/微生物共生网络/弗兰克氏菌属Key words
H.rhamnoides subsp.mongonica/H.rhamnoides subsp.sinensis/compartment niche/microbial symbiotic network/Frankia分类
农业科技引用本文复制引用
刘宏,段爱国,何彩云,张建国..中国沙棘和蒙古沙棘不同区室细菌微生物组特征与溯源分析[J].林业科学研究,2025,38(2):34-47,14.基金项目
沙棘优异种质创制与优质高产良种选育(2022YFD2200401-5)国家级 （2022YFD2200401-5）
中国林业科学研究院中央非公益性研究所基本科研业务费(CAFYBB2020SZ001-2)区市级 （CAFYBB2020SZ001-2）
