林业科学研究2025，Vol.38Issue(2)：67-77,11.DOI:10.12403/j.1001-1498.20240449
侧柏古树树洞微生物群落的结构及功能分析
Structure and Functional Analysis of Microbial Communities in the Tree Hollows of Ancient Platycladus orientalis Trees
摘要
Abstract
[Objective]To understand the types of pathogens causing cavity rot in ancient Platycladus ori-entalis L.trees and understand their metabolic functions,so as to provide a theoretical basis for the com-prehensive prevention and control of cavity rot in these ancient trees.[Methods]This study utilized physiological assays and metagenomic sequencing technology to analyze the microbial community char-acteristics and related functions of 100-year-old healthy cavity(JK100),100-,300-,and 700-year-old P.ori-entalis rotting cavities(SD100,SD300,and SD700).[Results]Results indicated that the cellulose content of the rotting cavities was lower than that of the control group,and gradually decreased with tree age of P.orientalis.In contrast,the activities of cellulase and pectinase were higher in the rotting cavities than those of the control group,and these activities increased with tree age of P.orientalis.In the rotting cavities of ancient P.orientalis trees,bacteria were the dominant microbial flora,followed by fungi.The dominant bac-terial phyla in the rotting cavities were Proteobacteria Gray&Herwig and Actinobacteria Goodfellow,while the dominant genera were Erysipelatoclostridium Yutin&Galperin and Paludifilum Frikha-Dammak.The most abundant species were Clostridium_innocuum Smith&King and Paludifilum_halophilum Frikha-Dam-mak.Furthermore,the abundances of Acidobacteria Thrash&Coates,Actinobacteria,Mycobacterium Lehmann&Neumann,Cellulomonas Bergey,Actinomycetospora_succinea Tamura from the bacterial do-mains,and Microthyrium_microscopicum Desm from the fungal domains were significantly higher in the rotting cavities than in the control group.The microbial communities in the rotting cavities also displayed higher diversity compared to the control."Carbohydrate metabolism","energy metabolism","cellular pro-cesses",etc.from the KEGG level 1 in the microbial community of P.orientalis tree holes were the main metabolic pathways that were up-regulated and enriched in the decayed tree cavity samples.Pathways such as peroxisome,phagosome,and bacterial chemotaxis from the KEGG level 3 were significantly up-regulated and enriched in the SD300 samples.[Conclusion]The microbial community structure and func-tion in the rotting cavities of ancient P.orientalis trees differ significantly from those in healthy cavities,with pronounced abilities for cellulose and pectin degradation.The results provide valuable microbiological in-sights that can inform the comprehensive management of decay in the hollows of ancient Platycladus ori-entalis tree.关键词
宏基因组学/侧柏/古树/微生物/功能差异Key words
Metagenomics/Platycladus orientalis/Ancient trees/Microorganisms/Functional differences分类
生物科学引用本文复制引用
吴昊宇,马晓燕,刘建锋,贾子瑞,常二梅..侧柏古树树洞微生物群落的结构及功能分析[J].林业科学研究,2025,38(2):67-77,11.基金项目
北京农学院省部级科研平台2023年度开放课题(BUAOP202301) （BUAOP202301）
中央级公益性科研院所基本科研业务费专项(CAFYBB2022ZA001) （CAFYBB2022ZA001）
