林业科学研究2025，Vol.38Issue(2)：106-115,10.DOI:10.12403/j.1001-1498.20240178
基于SSR标记的粗皮桉群体遗传多样性和群体结构分析
Genetic Diversity and Population Structure Analysis of Eucalyptus pellita F.Muell.Populations Using SSR Markers
摘要
Abstract
[Objective]The genetic diversity and population structure of Eucalyptus pellita F.Muell.were in-vestigated using simple sequence repeats(SSR)molecular markers to provide valuable insights for ger-mplasm management and breeding.[Method]This study utilized a provenance/progeny trial consisting of 18 populations of E.pellita originating from Indonesia,Papua New Guinea and Australia.A total of 18 SSR markers were utilized to examine the genetic diversity and population structure.Analytical methods in-cluded analysis of molecular variance analysis(AMOVA),cluster analysis and Bayesian model-based clus-tering method.[Result]A total of 187 alleles(Na)were detected across the 18 SSR markers,with an aver-age effective allele number(Ne)of 5.033.The observed heterozygosity(Ho)and expected heterozygosity(He)were 0.712 and 0.660,respectively.Polymorphism information content(PIC),Shannon's information Index(I)and Nei's genetic diversity index(H)were 0.753,1.338 and 0.777,respectively,indicating consid-erable genetic diversity within the E.pellita populations.The average genetic differentiation index(Fst)and gene flow(Nm)were 0.106,and 1.610,respectively.And the average genetic diversity of E.pellita popula-tions was high,with the highest levels found in WH,WD,SC,NC,NI populations in Australia,and MW populations in Papua New Guinea.AMOVA analysis revealed that 90%of the variation occurred at the in-tra-population level,while the remaining 10%was observed at the inter-population level.Population struc-ture analysis divided the 18 populations into two distinct groups:populations from Indonesia and Papua New Guinea formed one group,showing significant differentiation from the Australian populations.This pattern suggests an island differentiation effect.[Conclusion]The study revealed moderate genetic differ-entiation and substantial intra-population genetic variation in E.pellita.The observed high genetic diversity suggests promising potential for tree breeding.Populations with high diversity should be prioritized in ger-mplasm management to enhance the development of core collections and novel varieties.These findings provide a robust foundation for future germplasm collection,selection,and breeding strategies aimed at optimizing the utilization of E.pellita resources.关键词
粗皮桉/SSR/遗传多样性/遗传分化Key words
Eucalyptus pellita/simple-sequence-repeat(SSR)/genetic diversity/genetic differentiation分类
农业科技引用本文复制引用
邢梦月,王平,赵海文,翁启杰,周长品,王非,李发根..基于SSR标记的粗皮桉群体遗传多样性和群体结构分析[J].林业科学研究,2025,38(2):106-115,10.基金项目
十四五国家重点研发计划课题(2022YFD2200203--2)国家级 （2022YFD2200203--2）
中国林科院基本科研业务费专项(CAFYBB2021ZA001) （CAFYBB2021ZA001）
