湖南农业大学学报（自然科学版）2025，Vol.51Issue(2)：1-5,35,6.
自然储存条件下水稻种子耐储藏性状QTL的定位
QTL mapping for seed storage tolerance trait in rice under natural conditions
摘要
Abstract
To screen new rice storage tolerance germplasm,seed of one recombinant inbred lines(RILs)population that had been in natural storage for 4 years and derived from the cross between japonica cultivar'Mowanggu'(MGW)and indica rice'CO39'was used to analyze the germination phenotype of seed from the population and to map QTLs responsible for seed storage tolerance trait by composite interval mapping.The results showed that,the phenotype of germination rate,germination potential,and germination index of 67%lines from the 101 RIL lines was declined to 0.The value of the 3 traits for other lines ranged from 0-82.67%,0-46.00%and 0-6.48 respectively.Only one line still keeps a high germination rate of 82.67%.QTL mapping yielded 3 QTLs responsible for germination traits,namely qGP7,qGE7 and qGI7.Among them,qGR7 was mapped in the interval of SSR marker RM6344 and RM5720 with a distance of 18.43 cM,qGP7 and qGI7 located in the same interval between SSR maker RM5672 and RM8261 with a distance of 35.74 cM.The LOD values of 3 QTLs ranged from 2.52-2.56,of which the phenotypic contribution rate was 12.02%-12.22%.关键词
水稻/耐储藏性/重组自交系群体/数量性状基因位点/基因定位Key words
rice/storage tolerance/recombinant inbred lines population/quantitative trait locus/gene mapping分类
农业科学引用本文复制引用
陈文凤,陈哲红,程计华,刘金灵,黄浩,丁晓雯,林晋军,姚威,陈倩茹,黄希来,刘雄伦,吴俊..自然储存条件下水稻种子耐储藏性状QTL的定位[J].湖南农业大学学报（自然科学版）,2025,51(2):1-5,35,6.基金项目
国家自然科学基金项目(31972256) （31972256）
湖南省2023十大技术攻关项目(2023NK1010) （2023NK1010）
苏州市科技计划项目(SNG2021003) （SNG2021003）
