湖南农业大学学报（自然科学版）2025，Vol.51Issue(2)：44-50,96,8.
山银花'金翠蕾'愈伤组织诱导及植物生长调节剂2,4-D对愈伤组织中绿原酸质量分数的影响
Effect of callus induction and plant growth regulator 2,4-D on callus chlorogenic acid mass fraction of Lonicera hypoglauca'Jincuilei'
摘要
Abstract
In order to construct the callus induction system of Lonicera hypoglauca'Jincuilei'and analyze the effect of plant growth regulator 2,4-dichlorophenoxyacetic acid(2,4-D)on the mass fraction of chlorogenic acid in callus,the young leaves of Lonicera hypoglauca'Jincuilei'were used as explants to induce callus.The effects of different mass concentrations of growth regulator combinations on callus growth were compared,and the effects of different mass concentrations of 2,4-D on the mass fraction of chlorogenic acid in callus and the expression of key genes involved in chlorogenic acid synthesis were analyzed.The results showed that with the combination of 0.6 mg/L kinetin+1.0 mg/L 2.4-dichlorophenoxyacetic acid+0.6 mg/L 6-benzyladenine,the callus growth of'Jincuilei'was the highest at 30 d,which was 5.505 g.The effect of 2,4-D on the mass fraction of chlorogenic acid in callus was promoted at low concentration and inhibited at high concentration.When the mass concentration of 2,4-D was 1 mg/L,the mass fraction of chlorogenic acid was the highest,which was 26.224 mg/g.2,4-D treatment could promote the expression of key genes HQT and CCoAOMT in chlorogenic acid synthesis.It shows that the application of appropriate amount of plant growth regulator 2,4-D can increase the chlorogenic acid mass fraction of Lonicera hypoglauca callus.关键词
山银花'金翠蕾'/愈伤组织/2,4-二氯苯氧乙酸/绿原酸Key words
Lonicera hypoglauca'Jincuilei'/callus/2,4-dichlorophenoxyacetic acid(2,4-D)/chlorogenic acid分类
农业科学引用本文复制引用
陈蓉,罗景鸣,胡辉亮,申莹莹,彭国平,饶力群,刘实,汪启明..山银花'金翠蕾'愈伤组织诱导及植物生长调节剂2,4-D对愈伤组织中绿原酸质量分数的影响[J].湖南农业大学学报（自然科学版）,2025,51(2):44-50,96,8.基金项目
湖南省隆回县科技专家服务团项目(S2023NCKJFP0072) （S2023NCKJFP0072）
湖南省高层次聚集工程创新创业人才项目(2021RC5006) （2021RC5006）
湖南省重点研发计划项目(2020NK2023) （2020NK2023）
