湖南农业大学学报（自然科学版）2025，Vol.51Issue(2)：80-88,9.
基于转录组学研究宁乡猪肌肉的生长发育
Study on muscle growth and development in Ningxiang pigs based on transcriptomics
摘要
Abstract
At the ages of 1,60,120,180,240,300,and 360 days(recorded as ND1,ND60,ND120,ND180,ND240,ND300,and ND360 respectively),the longissimus dorsi muscle of 3 Ningxiang pigs were respectively selected for transcriptome sequencing analysis.An enrichment analysis was performed on the differentially expressed genes(DEGs)screened from 9 comparison groups.The levels of DEGs were verified using real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The results showed that 1 982,245,131,311,26,84,2 257,1 377,and 2 654 DEGs were identified in ND60 vs(short for versus)ND1,ND120 vs ND60,ND180 vs ND120,ND240 vs ND180,ND300 vs ND240,ND360 vs ND300,ND180 vs ND1,ND360 vs ND180,and ND360 vs ND1 comparison groups,respectively.Gene ontology(GO)analysis indicated that the DEGs were mainly involved in muscle contraction,skeletal muscle tissue development,and calcium ion binding processes.Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis showed that the DEGs were mainly enriched in signaling pathways such as gluconeogenesis/glycolysis,PI3K-Akt,and MAPK.The qRT-PCR verification of the DEGs showed the expression trends were consistent with the sequencing results.Through transcriptome sequencing and experimental verification,we found that THBS3 was downregulated in the ND180 vs ND1 and ND360 vs ND1 comparison group,and THBS4 was significantly downregulated in ND180 vs ND1 and upregulated in ND360 vs ND180,indicating the expression patterns of THBS3 and THBS4 were opposite to the growth and development pattern of pigs.It is speculated that those may negatively regulate the growth and development of skeletal muscles.关键词
宁乡猪/背最长肌/转录组/差异表达基因/GO分析/KEGG通路分析/实时荧光定量PCRKey words
Ningxiang pig/longissimus dorsi muscle/transcriptome/differentially expressed gene(DEG)/GO analysis/KEGG pathway analysis/real-time fluorescence quantitative PCR(qRT-PCR)分类
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张维,张四阳,彭英林,刘莹莹,崔清明,任慧波,陈晨,李华丽,胡雄贵,朱吉,杨仕柳,李述初..基于转录组学研究宁乡猪肌肉的生长发育[J].湖南农业大学学报（自然科学版）,2025,51(2):80-88,9.基金项目
湖南省自然科学基金项目(2022JJ30317、2021JJ30386) （2022JJ30317、2021JJ30386）
湖南省创新平台与人才计划项目(2022NK4145)、湖南省科技重大专项(2021NK1009) （2022NK4145）
湖南省科技成果转化与产业化计划项目(2022GK4016) （2022GK4016）
