临床神经外科杂志2025，Vol.22Issue(2)：181-187,192,8.DOI:10.3969/j.issn.1672-7770.2025.02.010
FMNL1对胶质瘤细胞增殖、迁移和侵袭的影响
Effect of FMNL1 on proliferation,migration and invasion of glioma cells
摘要
Abstract
Objective To investigate the effect of FMNL1 on the proliferation,migration and invasion of glioma cells,and to provide a new potential target for the clinical treatment of glioma patients.Methods The Cancer Genome Atlas(TCGA)and Chinese Glioma Genome Atlas(CGGA)databases were used to explore the relationship between FMNL1 and glioma grade and prognosis of patients.The effect of FMNL1 on the proliferation,migration and invasion of glioma cells was investigated by CCK-8,cell scratch and Transwell migration and invasion function experiments.Western blot(WB)was used to detect the epithelial mesenchymal transition(EMT)in U251-shFMNL1,U251-OEFMNL1,U87-OEFMNL1 and LN229-OEFMNL1 cell lines Transition(EMT),and to explore the effect of FMNL1 on EMT.Results The database of clinical samples showed that the expression level of FMNL1 was positively correlated with the WHO grade of glioma and significantly affected the survival time of glioma patients.Knocking down or up-regulating the expression of FMNL1 had a significant effect on the proliferation,migration,and invasive ability of glioma cells.The results of WB showed that knocking down the expression of FMNL1 significantly elevated the expression of E-cadherin expression was significantly elevated,while the expression of N-cadherin and Vimentin was significantly decreased after knocking down the expression of FMNL1.After up-regulating the expression of FMNL1,the expression of E-cadherin was significantly decreased,while the expression of N-cadherin and Vimentin was significantly increased.Conclusions The expression level of FMNL1 is closely related to the WHO grade and the prognosis of glioma patients.As a cancer promoting factor,FMNL1 can significantly improve the proliferation,migration and invasion ability of glioma cells,and at the same time FMNL1 promotes the EMT process of glioma cells.关键词
胶质瘤/FMNL1/增殖/迁移/侵袭/上皮间质转化Key words
glioma/FMNL1/proliferation/migration/invasion/EMT分类
医药卫生引用本文复制引用
陈锦娣,于莉,陈亮,胡陵珊,秦军..FMNL1对胶质瘤细胞增殖、迁移和侵袭的影响[J].临床神经外科杂志,2025,22(2):181-187,192,8.基金项目
湖北医药学院研究生科技创新项目(YC2023041) （YC2023041）
