宁夏农林科技2025，Vol.66Issue(3)：25-30,52,7.DOI:10.3969/j.issn.1002-204x.2025.03.006
不同产地枸杞类胡萝卜素HPLC指纹图谱建立及其抗氧化活性谱-效关系研究
Establishment of HPLC Fingerprints of Carotenoids of Lycium barbarum from Different Places and A Study on Relationship between Antioxidant Activity Spectrum and Effect
摘要
Abstract
HPLC fingerprints of carotenoids of"Ningqi No.1"Lycium barbarum from different origin(S1-S7)were studied,and the relationship between their spectral activity and antioxidant activity was studied.The carotenoid fingerprints of Lycium barbarum from different regions were established by the Similarity Evaluation System of TCM Fingerprint(2012 edition),and the common peak identification,similarity evaluation and cluster analysis were carried out.Using DPPH and hydroxyl radical clearance as antioxidant indexes,the spectrum-effect relationship between carotenoids and antioxidant indexes of Lycium barb arum was analyzed by grey correlation degree method.The results showed that there were 10 common peaks of carotenoids in Lycium barbarum,and the peaks 1,2,4 and 10 were identified as zeaxanthin,β-cryptoxanthin,β-carotenoid and zeaxanthin dipalmitate,respectively.The composition of carotenoids was similar in the 7 producing areas,and the highest content was zeaxanthin dipalmitate.The antioxidant activity of Lycium barb arum from different producing areas was significantly different.The antioxidant capacity of carotenoid of"Ningqi No.1"from Zhongning,Ningxia(S1),Xingren,Ningxia(S2)and Golmu,Qinghai(S4)was significantly higher than that from the other four producing areas.The spectrum-effect relationship analysis indicated that,except for peak 1,the correlation coefficients between the other common peaks and the DPPH radical clearance rate were all greater than 0.6,and the correlation coefficient between peak 10 and the DPPH radical clearancerate was greater than 0.8.The correlation coefficients between the 10 common peaks and the hydroxyl radical clearance rate ranged from 0.60 to 0.72,and the correlation coefficient between peak 10 and the hydroxyl radical clearance rate was the highest,reaching 0.72.This suggests that the basis for the antioxidant effect of Lycium barbarum carotenoids may be the combined action of the chemical components represented by each common peak,among which the component represented by peak 10-zeaxanthin dipalmitate-may be the main active component of the antioxidant effect of Lycium barbarum carotenoids.关键词
枸杞/类胡萝卜素/高效液相色谱/抗氧化活性/谱-效关系Key words
Lycium barbarum/Carotenoids/High performance liquid chromatography(HPLC)/Antioxidant activity/Spectrum-effect relationship分类
农业科技引用本文复制引用
李瑞清,金波,闫亚美,姜新,禄璐,张渌淘,米佳,刘春红,冉林武..不同产地枸杞类胡萝卜素HPLC指纹图谱建立及其抗氧化活性谱-效关系研究[J].宁夏农林科技,2025,66(3):25-30,52,7.基金项目
宁夏回族自治区农业科技自主创新资金项目(NGSB-2021-2-06),宁夏创新群体基金(2024AAC01003),宁夏自然科学基金(2023AAC03168). （NGSB-2021-2-06）
