中国海洋大学学报（自然科学版）2025，Vol.55Issue(5)：128-137,10.DOI:10.16441/j.cnki.hdxb.20240136
炭基菌剂促进Cr(Ⅵ)还原和Cr(Ⅲ)固定的协同效能及机制研究
Study of Synergistic Effect and Mechanism of Biochar Based Bacterial Agent in Promoting the Reduction of Cr(Ⅵ)and the Fixation of Cr(Ⅲ)
摘要
Abstract
Microbial reduction plays an important role in the remediation of Cr(Ⅵ)pollution,but there are problems of low efficiency and microbial loss.This study developed an amine-modified biochar(PBC)from rice husks,onto which Cr(Ⅵ)reducing bacteria were loaded to create a microbial biochar composite(MPBC).The synergistic effects and mechanisms of PBC and Cr(Ⅵ)reducing bacteria on the reduction of Cr(Ⅵ)to Cr(Ⅲ)and the immobilization of Cr(Ⅲ)were investigated.Results indicated that the quantity of adsorbed microbes on the PBC surface was 4.3 times that on the original biochar,significantly reducing microbial loss.The experimental results for Cr(Ⅵ)removal show that the remov-al rates of chromium-reducing bacteria,PBC,and MPBC in the first 6 hours of the experiment were 1.6,5.9,and 11.7 mg·(L·h)-1,respectively,demonstrating a strong synergistic chromium removal effect between PBC and the bacteria,with a synergy coefficient of 49.6％.Product analysis revealed that 75.9％ of chromium(Cr)was immobilized on the MPBC surface,with XPS analysis of MPBC con-firming the predominance of Cr(Ⅲ)fixation.The pathway for Cr removal by the biochar composite in-volves the amino functional groups of MPBC capturing Cr(Ⅵ),facilitating the reduction of Cr(Ⅵ)by the microbial biochar composite,and promoting the release of extracellular chromium reductase,and re-ducing Cr(Ⅵ).The immobilization of Cr(Ⅲ)is achieved through amino complexation and extracellular polymeric substances,thereby enhancing the synergistic remediation capability of amine-modified bio-char and Cr(Ⅵ)reducing bacteria for chromium contamination.This study offers a novel approach for the simultaneous reduction of Cr(Ⅵ)and immobilization of Cr(Ⅲ)in chromium-contaminated environ-ments.关键词
生物炭/胺基改性/铬还原菌/还原/协同效应Key words
biochar/amine-modified/Cr(Ⅵ)reducing bacteria/reduction/synergistic effect分类
资源环境引用本文复制引用
陈友媛,李浩,刘乐成,寇晗,王磊..炭基菌剂促进Cr(Ⅵ)还原和Cr(Ⅲ)固定的协同效能及机制研究[J].中国海洋大学学报（自然科学版）,2025,55(5):128-137,10.基金项目
国家自然科学基金青年基金项目(42207060) （42207060）
中国博士后科学基金项目(2022M722980)资助Supported by the National Natural Science Youth Foundation of China(42207060) （2022M722980）
the China Postdoctoral Science Foundation(2022M722980) （2022M722980）
