中国海洋大学学报（自然科学版）2025，Vol.55Issue(5)：138-148,11.DOI:10.16441/j.cnki.hdxb.20240138
Cu(Ⅱ)、Ni(Ⅱ)对厌氧氨氧化脱氮性能及微生物影响研究
The Effects of Cu(Ⅱ)and Ni(Ⅱ)on Nitrogen Removal Performance and Microorganisms in ANAMMOX-UASB Reactor
摘要
Abstract
Cu(Ⅱ)and Ni(Ⅱ)are widely distributed in various industrial wastewater.To promote the application of the anaerobic ammonium oxidation(anammox)process for treating ammonium-rich indus-trial wastewater,the effects of Cu(Ⅱ)and Ni(Ⅱ)on nitrogen removal performance and microorgan-isms in ANAMMOX-UASB reactors were investigated.The enzyme activities of nitrate reductase(NR),nitrite reductase(NiR),hydrazine synthase(HZS)and hydrazine oxidase(HZO)were ana-lyzed,and the inhibition mechanisms of Cu(Ⅱ)and Ni(Ⅱ)on anammox were revealed.The results showed that the inhibition thresholds of Cu(Ⅱ)and Ni(Ⅱ)on anammox were 2.0 and 1.0 mg·L-1,respectively,with the nitrogen loading rate(NLR)of 1.50 kg·m-3·d-1.2.0 mg·L-1 Cu(Ⅱ)and 1.0 mg·L-1 Ni(Ⅱ)decreased the specific anammox activity(SAA)by 56.21％and 58.71％,respec-tively,while the ANAMMOX-UASB reactors maintained stable performances with nitrogen removal ef-ficiencies(NRE)of 81.65％and 83.24％.However,4.0 mg·L-1 Cu(Ⅱ)and 2.0 mg·L-1Ni(Ⅱ)decreased sharply the NRE of ANAMMOX-UASB reactors to 17.09％and 22.07％,respectively.With-out adding Cu(Ⅱ)and Ni(Ⅱ),the nitrogen removal performances of ANAMMOX-UASB reactors re-covered within 20 and 54 days,respectively,but SAA was only recovered to 5.62 and 4.90 mg·g-1·h-1,42.29％and 37.81％of the initial values.The inhibition of Ni(Ⅱ)on the anammox was mainly attribu-ted to the adverse effect on NiR enzyme activity,and 2.0 mg·L-1 Ni(Ⅱ)reduced NiR activity by 40.00％.In contrast,Cu(Ⅱ)did not substantially inhibit the four anammox functional enzymes,with 4.0 mg·L-1 Cu(Ⅱ)only reducing HZS activity by 10.00％.Secreting a substantial amount of extra-cellular polymeric substance(EPS)for Anaerobic ammounium oxidizing bacteria(AnAOBs)was the effective method of alleviating Cu(Ⅱ)stress,yet it was not resistant to Ni(Ⅱ)stress.Additionally,the niche between Candidatus Brocadia and Candidatus Jettenia in ANAMMOX-UASB reactors shif-ted under the Cu(Ⅱ)and Ni(Ⅱ)stress,and Candidatus Jettenia displayed greater tolerance to Cu(Ⅱ)and Ni(Ⅱ).关键词
厌氧氨氧化/Cu(Ⅱ)/Ni(Ⅱ)/酶活性Key words
anammox/Cu(Ⅱ)/Ni(Ⅱ)/enzyme activity分类
资源环境引用本文复制引用
李登志,刘海涛,伍洋伯,崔锡钦,季军远..Cu(Ⅱ)、Ni(Ⅱ)对厌氧氨氧化脱氮性能及微生物影响研究[J].中国海洋大学学报（自然科学版）,2025,55(5):138-148,11.基金项目
国家自然科学基金项目(51978636)资助Supported by the National Natural Science Foundation of China(51978636) （51978636）
