皮革科学与工程2025，Vol.35Issue(2)：23-29,7.DOI:10.12472/j.issn.1004-7964.202400119
EGCG对Ⅰ型胶原酶结构及水解活性的影响
Effect of EGCG on the Structure and Activity of Type Ⅰ Collagenase
摘要
Abstract
In the tanning industry,enzymatic unhairing process show the risk of collagen damage on the grain.In order to ensure the quality of leather,it is importance to regulation of collagenase activity during enzymatic unhairing process.Type Ⅰ collagenase was mixed with epigallocatechin gallate(EGCG),and its hydrolytic activity to collagen was measured,in addition,the structural effects of EGCG on type Ⅰ collagenase were explored by ultraviolet spectro-scopy,fluorescence spectroscopy,and molecular docking technology.The results showed that EGCG concentration of 100 μmol/L could significantly inhibit the collagen hydrolysis activity of type Ⅰ collagenase(86.06±0.14)％.EGCG can catalyze Zn(Ⅱ)ion coordination with the active center of type Ⅰ collagenase,and has hydrogen bonds and hydrophobic interactions with amino acid residues in the protease molecule,resulting in the fluorescence quenching of proteases and decreased collagenase activity.This work provides a way for the screening and design of collagenase activity regulators.关键词
Ⅰ型胶原酶/EGCG/分子对接/荧光光谱/酶活力调控Key words
Type Ⅰ collagenase/EGCG/molecular docking/fluorescence spectrum/regulation of enzyme activity分类
轻工纺织引用本文复制引用
周雪伟,伍思进,程海明..EGCG对Ⅰ型胶原酶结构及水解活性的影响[J].皮革科学与工程,2025,35(2):23-29,7.基金项目
国家自然科学基金面上项目(22178231) （22178231）
