四川农业大学学报2025，Vol.43Issue(2)：296-305,10.DOI:10.16036/j.issn.1000-2650.202412527
芦笋EXP基因家族鉴定及其在茎伸长阶段表达分析
Identification of EXP Gene Family in Asparagus(Asparagus officinalis L.)and Its Expression Analysis During Stem Elongation
摘要
Abstract
[Objective]To perform a genome-wide identification of the EXP gene family members in as-paragus and investigate the mechanisms of EXP genes involvement involved in the growth of asparagus tender stems.[Method]Bioinformatics methods were used to identify and analyze the EXP gene family members in the asparagus genome,and quantitative real-time PCR(qRT-PCR)was utilized to validate the expression patterns of AoEXPs at different growth stages.[Result]The asparagus genome comprises 23 EXP gene members,which are randomly distributed across eight chromosomes,with all encoded pro-teins localized to the cell wall.Structural and evolutionary analysis showed that these 23 AoEXPs can be classified into three subfamilies:EXPA,EXPB,and EXPLB.All genes possess three common motif structures,and their promoter regions contain varying numbers of cis acting elements related to plant hor-mones and cell division elongation.The transcriptome data revealed that out of 23 EXP genes,17 Ao-EXPs were expressed during asparagus stem elongation,with 10 EXP members were differentially ex-pressed at different developmental stages.qRT-PCR validation revealed that the AoEXPs genes had their own specific spatio-temporal expression patterns,which are consistent with the transcriptome expression abundance.For example,the four genes AoEXPA5,AoEXPA7,AoEXPA10 and AoEXPB1 showed a gradual decrease in expression levels with stem elongation.It was hypothesized that these 10 genes may be involved in the process of regulating the elongation and growth of asparagus stems.[Conclusion]This study preliminarily identified and characterized on the members of the asparagus EXP gene family,lay-ing an important foundation for future research on the functional exploration of AoEXP genes.关键词
芦笋/EXP家族/序列分析/茎伸长Key words
asparagus/EXP gene family/sequence analysis/stem elongation分类
农业科技引用本文复制引用
李威龙,贺茂林,仇公凯,李欢,李梦瑶,谢婉,鲜登宇,岳诚,郑阳霞..芦笋EXP基因家族鉴定及其在茎伸长阶段表达分析[J].四川农业大学学报,2025,43(2):296-305,10.基金项目
国家现代农业产业技术体系四川特色蔬菜创新团队(SCCXTD-2024-22). （SCCXTD-2024-22）
