四川农业大学学报2025，Vol.43Issue(2)：350-364,15.DOI:10.16036/j.issn.1000-2650.202409518
'黑果枸杞'杂交F1代群体遗传结构及其农艺性状关联分析
Correlation Analysis of Genetic Structure and Agronomic Traits on F1 Hybrids of Lycium ruthenicum Murr.
摘要
Abstract
[Objective]This study aimed to identify molecular markers related to important agronomic traits in Lycium ruthenicum Murr.to provide a reference for breeding new varieties and germplasm innova-tion.[Method]A hybrid F1 population of 227 L.ruthenicum samples was used as experimental materi-als.Based on the diversity,correlation and principal component analysis of 17 important agronomic traits,combined with genetic diversity and population structure analysis using 19 SSR primers.The MLM model in Tassel 3.0 software was used to analyze the association between arterial traits and SSR markers,and the markers were selected for new variety breeding.[Result]The variation coefficients of 17 pheno-typic traits in 227 F1 hybrids exhibited a coefficients of variation(CV)exceeding 20%,with Shannon's diversity index(H')all above 6.5.Correlation analysis showed that the leaf area was significantly posi-tively correlated with leaf width and leaf length(P<0.01),while the number of immature fruits was sig-nificantly positively correlated with fruit stalk length(P<0.01).PCA identified six principal components with a cumulative contribution rate of 88.78%,among which nine key traits were determined:leaf width(-0.79),leaf length(-0.76),fruit longitudinal diameter(0.75),fruit transverse diameter(0.73),num-ber of main branches per plant fruit(0.72),branch base diameter(0.63),average fruit weight per fruit(0.61),branch length(0.57),anthocyanin content(0.41).Polymorphism information content(PIC)with a mean of 0.758,and the total genetic differentiation index(Fst)was 0.010(P<0.05),indicating that ge-netic variation primarily originated within the hybrid population.Genetic structure and clustering analysis divided the samples into six subgroups.Five SSR markers were highly correlated with leaf width,leaf length,leaf area and other growth traits by association analysis(MLM model),and the explanation rate of phenotypic variation was over 10.00%.Notably,same markers were associated with multiple traits,and same traits were linked to multiple markers.[Conclusion]L.ruthenicum exhibits extensive genetic diversity in branches,leaves and fruit traits.The five identified markers demonstrate"pleiotropic"ef-fect,suggesting their potential utility in marker-assisted breeding.关键词
黑果枸杞/农艺性状/SSR/遗传结构/关联分析Key words
Lycium ruthenicum Murr./quality traits/SSR/genetic diversity/association analysis分类
农业科技引用本文复制引用
戴国礼,米佳,张波,尹跃,秦垦,何昕孺..'黑果枸杞'杂交F1代群体遗传结构及其农艺性状关联分析[J].四川农业大学学报,2025,43(2):350-364,15.基金项目
国家自然科学基金(32060360) （32060360）
宁夏回族自治区重点研发计划(2020BBF02006、2021BEF02005). （2020BBF02006、2021BEF02005）
