口腔颌面外科杂志2025，Vol.35Issue(2)：91-97,7.DOI:10.12439/kqhm.1005-4979.2025.02.002
Zfp260对牙周炎破骨细胞分化影响的实验研究
Experimental study of the effect of Zfp260 on the differentiation of osteoclasts in periodontitis
摘要
Abstract
Objective:To research the role of zinc finger protein 260(Zfp260)in osteoclast differentiation under the periodontitis microenvironment.Methods:Small interfering RNA(siRNA)was transfected into the monocyte/macrophage cell line RAW264.7.The transfected cells were induced into osteoclasts by receptor activator of nuclear factor κB ligand(RANKL)for 7 days.Differentiated cells were harvested for tartrate resistant acid phosphatase(TRAP)staining.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expression level of osteoclast differentiation-related genes.In vivo,experimental periodontitis models were established in Zfp260 flox/flox mice(Control group)and Zfp260 flox/flox Lyz2-cre-myeloid cell conditional Zfp260-knockout mice(Zfp260 cko,Experimental group).The Micro-CT scan and histological staining were used to analyze alveolar bone resorption.Results:Zfp260 was up-regulated during osteoclast genesis.The knockdown of Zfp260 inhibited the differentiation of RAW264.7 cells into osteoclasts,and the expression of osteoclast-related factors was down-regulated(P<0.05).In vivo,the bone resorption was significantly decreased on the experimental side in Zfp260CKO mice.Conclusion:Zfp260 regulates the osteoclast differentiation ability,which will provide a new target for solving periodontitis.关键词
牙周炎/牙槽骨吸收/破骨细胞/锌指蛋白260Key words
periodontitis/alveolar bone resorption/osteoclasts/zinc finger protein 260分类
口腔医学引用本文复制引用
贡若男,王佐林..Zfp260对牙周炎破骨细胞分化影响的实验研究[J].口腔颌面外科杂志,2025,35(2):91-97,7.基金项目
科技部重点研发专项(2018YFE0202200) （2018YFE0202200）
国家自然科学基金(81670962) （81670962）
