农业生物技术学报2025，Vol.33Issue(5)：958-971,14.DOI:10.3969/j.issn.1674-7968.2025.05.002
基于代谢组学技术分析3种颜色绿豆的呈色物质
Analysis of Present-color Materials in 3 Colored Mung Beans(Vigna radiate)Based on Metabolomics Techniques
摘要
Abstract
The diversity of color in mung bean(Vigna radiate)seed coat is one of the important phenotypic characteristics.Flavonoids are the key substances affecting the color of mung bean seed coat,and they have important medicinal value.There are great differences in nutritional and economic value for mung beans with different seed coat color.'Zhangjiakou yellow mung bean'(ZH),'Jin mung bean 8'(JL8)and'Ji black green 0506'(JH)were used as experimental materials.The phenotypic parameters of mung bean were determined by non-contact spectrophotometer.The metabolites of mung bean seeds were detected and analyzed by widely targeted metabolomics technology.The key differential metabolites were screened by K-means clustering analysis and the correlation analysis was done with phenotypic parameters.The molecular mechanism of the nutritional value and colour formation were explored for the mung beans with different seed coat color.The 537 metabolites,218 differential metabolites and 15 common differential metabolites were detected in the 3 mung beans.ZH shows significant differences in the distribution and accumulation of secondary metabolites,especially flavonoids,compared to JL8 and JH.Metabolome data analysis showed that selphinidin 3-glucoside and quercetin 3-O-β-D-glucosyl-(1→2)-rhamnoside might be the substances that cause the black color of the seed coat of JH.The luteolin 7-rutinoside,vitexin 2"-glucoside,nudifloramide and i-inositol were the causes of green accumulation in mung bean seed coat by the analysis of K-means clustering and correlation analysis.This study provides essential data for further revealing the pigments and the difference of nutrients of mung beans with different seed coat colors at the molecular level.关键词
绿豆/代谢组学/种皮颜色/相关性/营养品质Key words
Mung bean/Metabolomics/Seed coat color/Correlation/Nutritional quality分类
农业科学引用本文复制引用
张鸣慧,王新妤,张丁,张振鑫,申慧芳,郭锋..基于代谢组学技术分析3种颜色绿豆的呈色物质[J].农业生物技术学报,2025,33(5):958-971,14.基金项目
山西省重点研发计划项目(201803D221020-3) （201803D221020-3）
