农业生物技术学报2025，Vol.33Issue(5)：999-1005,7.DOI:10.3969/j.issn.1674-7968.2025.05.005
大葱SVP基因的克隆及其对温度与干旱胁迫的响应
SVP Gene Cloning and Its Response to Temperature and Drought Stress in Green Onions(Allium fistulosum)
摘要
Abstract
SVP(short vegetative phase)is one of the important genes in the regulation of plant flowering.In this study,the AfSVP gene(GenBank No.AfisC4G02946)of green onion(Allium fistulosum)was cloned.Its CDS was 744 bp and encoded for 247 amino acids.Bioinformatics analysis revealed that the AfSVP protein had a molecular weight of 60.35 kD and was a hydrophobic protein.Sequence alignment across multiple species demonstrated it was high degree of conservation;Following the introduction of the AfSVP gene into Nicotiana benthamiana protoplasts,laser-confocal microscopy was employed to visualize the protein's subcellular localization.The results showed that AfSVP was present in both the cytoplasm and the nucleus.Tissue-specific expression analysis further indicated that the expression level of AfSVP was significantly higher in flowers compared to other tissues(P＜0.05).The analysis of high,low temperature and drought stress response showed that the expression of AfSVP reached the highest at 12 h under high temperature stress,at 72 h under low temperature stress and at 12 h under drought stress(P＜0.05).This study offers a reference for further research on how plants adapt to environmental changes.关键词
大葱/SVP(short vegetative phase)/环境胁迫/表达分析/生物信息学分析Key words
Green onions/SVP(short vegetative phase)/Environmental stress/Expression analysis/Bioinformatics analysis分类
农业科技引用本文复制引用
刘敏,康屹锋,张宇辰,王永勤..大葱SVP基因的克隆及其对温度与干旱胁迫的响应[J].农业生物技术学报,2025,33(5):999-1005,7.基金项目
国家自然科学基金(31972409) （31972409）
北京市农林科学院科技创新能力项目(KJCX20200113) （KJCX20200113）
北京市农林科学院种质资源项目(KJCX20230126) （KJCX20230126）
北京市农林科学院蔬菜研究所改革与发展项目(KYCX202303) （KYCX202303）
