农业生物技术学报2025，Vol.33Issue(5)：1006-1017,12.DOI:10.3969/j.issn.1674-7968.2025.05.006
百合光信号转导调控因子LhSPA1基因的克隆和表达分析
Cloning and Expression Analysis of Light Signal Transduction Regulatory Factor LhSPA1 Gene in Lily
摘要
Abstract
Anthocyanin is one of the key substances determining the color of lily(Lilium brownii var.viridulum)flowers,and its biosynthesis is susceptible to various environmental factors.SPA1(suppressor of phyA-105)transcription factor plays a crucial role in regulating plant light signal transduction.To further understand the role of LhSPA1 in the regulation of anthocyanin induction in oriental hybrid lily(Lilium oriental hybrid),this study utilized'Sunny Martinique'cultivar as material.The differences of anthocyanin content in lily buds treated with different light treatments were compared,and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to detect the perianth slices of flower buds in the complete color development period.Combined with the previous transcriptome data,LhSPA1 was cloned and then subjected to bioinformatics analysis;and the expression levels of genes related to anthocyanin biosynthesis under different light conditions were analyzed using qRT-PCR.The results showed that the main color components in the tepals of'Sunny Martinique'were cyanidin and pelargonidin.Shading treatment reduced the accumulation of anthocyanin in lily buds.The results of gene expression analysis showed that the expression of anthocyanin biosynthesis-related genes was down-regulated under shading treatment,but the expression of LhSPA1 was significantly higher than that under normal light conditions.The coding sequence length of LhSPA1(GenBank No.PQ499086)was 2 931 bp,encoding 976 amino acids.Phylogenetic tree analysis showed that LhSPA1 protein was closely related to ZmSPA1 protein in monocotyledonous plant.Sequence analysis revealed that LhSPA1 protein shared conserved domains with SPA1 protein from other species.Subcellular localization results revealed that the fluorescence signal of LhSPA1 protein was located in the nucleus of Nicotiana tabacum.Transient overexpression of LhSPA1 and constitutive photomorphogenic 1(LhCOP1)in lily petals resulted in light lily flower color.In conclusion,it was speculated that LhSPA1 played an important role in light-induced anthocyanin biosynthesis in lily.This study lays a foundation for analyzing the molecular mechanism of light signal regulating lily anthocyanin biosynthesis pathway.关键词
东方百合/花青苷/光照/SPA1/基因表达Key words
Lilium oriental hybrid/Anthocyanin/Light/SPA1/Gene expression分类
农业科技引用本文复制引用
黄丽,杨捷,吕博,宋居荣,陈锋,过聪,杨园园,向发云,许本波..百合光信号转导调控因子LhSPA1基因的克隆和表达分析[J].农业生物技术学报,2025,33(5):1006-1017,12.基金项目
湖北省重点研发计划(2024BBB022) （2024BBB022）
湖北省国际科技合作项目(2024EHA022) （2024EHA022）
湖北省农业科技创新中心项目(2024-620-000-001-008) （2024-620-000-001-008）
中国博士后科学基金(2023M731036) （2023M731036）
