中南医学科学杂志2025，Vol.53Issue(2)：205-210,6.DOI:10.15972/j.cnki.43-1509/r.2025.02.004
NEAT1/miR-27b轴促进LPS诱导ARDS小鼠肺损伤和炎症
NEAT1/miR-27b axis promotes LPS-induced lung injury and inflammation in ARDS mice
摘要
Abstract
Aim To investigate the role and mechanism of long non-coding RNA(lncRNA)nuclear-enriched abundant tran-script 1(NEAT1)in LPS-induced lung injury and inflammation in acute respiratory distress syndrome(ARDS).Methods 40 C57BL/6 mice were randomly divided into control group,ARDS group,sh-NC group and sh-NEAT1 group.ARDS models were established in all groups except the control group.qRT-PCR,HE staining,TUNEL were used to detect the expression level of NEAT1,tissue damage and apoptotic cells in lung tissue.The expressions of tumor necrosis factor(TNF)-α,interleukin(IL)-6 and IL-10 in lung tissue were detected by enzyme-linked immunosorbent assay.Small airway epithelial cells(SAEC)of hu-man lung epithelial cell line were divided into normal group,LPS group,LPS+sh-NEAT1 group and LPS+sh-NEATl+miR-27b inhibi-tor group.Cell viability was detected by CCK-8 method,and apoptosis was detected by flow cytometry.The interactions between NEAT1,miR-27b and nuclear factor-erythroid-2-related factor 2(Nrf2)were assessed by using dual luciferase reporter gene assay.Results The expression level of NEAT1 was increased in LPS-induced ARDS mouse model and SAEC.sh-NEAT1 resulted in in-creased cell viability and decreased apoptosis,leading to protective effect against LPS-induced cell damage.sh-NEAT1 inhibited LPS-induced neutrophil number,myeloperoxidase activity,and production of inflammatory cytokines IL-6,and TNF-α.The silencing of miR-27b reversed the effects of sh-NEAT1 on LPS-induced damage in SAEC.sh-NEAT1 reduced the expression level of Nrf2 in LPS-induced SAEC.Co-transfection of sh-NEAT1 with miR-27b inhibitor reversed the effect of sh-MINCR on the expression of Nrf2.Conclusion NEAT1 promotes LPS-induced lung injury and inflammation in ARDS,which may be related to the inhibition of miR-27b and up-regulat on of Nrf2.关键词
lncRNA NEAT1/miR-27b/Nrf2/急性呼吸窘迫综合征/小鼠Key words
lncRNA NEAT1/miR-27b/Nrf2/ARDS/mice分类
医药卫生引用本文复制引用
张小雷..NEAT1/miR-27b轴促进LPS诱导ARDS小鼠肺损伤和炎症[J].中南医学科学杂志,2025,53(2):205-210,6.基金项目
湖南省创新型省份建设专项(S2021JJKWLH0129) （S2021JJKWLH0129）
