农业生物技术学报2025，Vol.33Issue(5)：1031-1043,13.DOI:10.3969/j.issn.1674-7968.2025.05.008
苦参查尔酮合酶(CHS)基因家族的鉴定与表达谱分析
Identification and Expression Profile Analysis of Chalcone Synthase(CHS)Gene Family in Sophora flavescens
摘要
Abstract
The root of Sophora flavescens is rich in flavonoid secondary metabolites.Chalcone synthase(CHS)is the first rate-limiting enzyme in the flavonoid biosynthesis pathway,and its characteristics and activity directly affect the biosynthesis and accumulation of secondary metabolites.To analyze the phylogenetic relationship of CHS genes in S.flavescens and their expression profiles,and explore their roles in flavonoid biosynthesis,this study identified 21 CHS family members in S.flavescens using bioinformatics methods.Gene duplication contributed greatly to this family expansion,and the duplicate SfCHS genes underwent strong purifying selection.Then,these CHS members were subdivided into 3 groups(Group Ⅰ,Ⅱand Ⅲ)based on phylogenetic analysis.CHS family were not conserved during the evolution,but these members belonging to the same group were conserved in gene structure and motif distribution.The SfCHS04 promoter region contained the highest number of cis-elements for stress resistance.Moreover,based on the expression level of SfCHS genes among different tissues(root,stem,leaf,and flower),majority genes were specifically expressed in flowers.Based on the co-expression network analysis for transcriptome and metabolome data of the pod development,SfCHS04 and SfCHS20 were located in the center of the network,and the main regulatory transcription factors included members of ERF,MYB,WRKY,and NAC families,which played important roles in the development of S.flavescens pods and the accumulation of flavonoids.Finally,the quantitative of SfCHS genes using qPCR technology,which revealed that distinct SfCHS genes showed significant variations in expression levels during root development and among multiple tissues at different growth stages.In summary,the results not only clarify the phylogeny and expression profile of SfCHS genes,but also provide reference value for understanding the molecular mechanism of flavonoid biosynthesis and improving the medicinal value of S.flavescens through molecular breeding methods.关键词
苦参/查尔酮合酶(CHS)/比较基因组学/黄酮/共表达网络Key words
Sophora flavescens/Chalcone synthase(CHS)/Comparative Genomics/Flavonoid/Coexpression network分类
农业科技引用本文复制引用
刘阿克,卢俊洁,刘林涛,牛天增,刘江天,宋慧芳,王茜..苦参查尔酮合酶(CHS)基因家族的鉴定与表达谱分析[J].农业生物技术学报,2025,33(5):1031-1043,13.基金项目
山西省基础研究计划(自由探索类)青年科学研究项目(20210302124145) （自由探索类）
山西省回国留学人员科研资助项目(2021-153) （2021-153）
山西省1331工程优势特色学科建设计划(1331KSC) （1331KSC）
