中南医学科学杂志2025，Vol.53Issue(2)：226-231,6.DOI:10.15972/j.cnki.43-1509/r.2025.02.008
上调miR-338-3p通过靶向MACC1抑制恶性黑色素瘤A375细胞增殖、迁移和侵袭
Upregulation of miR-338-3p inhibits the proliferation,migration and invasion of malignant melanoma A375 cells by targeting MACC1
摘要
Abstract
Aim To investigate the molecular mechanisms of miR-338-3p upregulation on proliferation,migration and inva-sion of malignant melanoma A375 cells.Methods GSE8401,GSE18509 melanoma datasets and 30 melanoma tissue specimens were included,and the expression of miR-338-3p,MET-associated in colon cancer 1(MACC1)in different tumour tissues were detec-ted.A375 cells were transfected with different vectors and then divided into miR-NC group(transfected with miR-338-3p overexpres-sion empty vector),Vector group(transfected with MACC1 overexpression empty vector),sh-NC group(transfected with knockdown MACC1 empty vector),sh-MACC1 group(transfected with knockdown MACC1),p-MACC1 group(transfected with overexpression MACC1),miR-338-3p mimic group(transfected with miR-338-3p mimic),and miR-338-3p mimic+p-MACC1 group(transfected with miR-338-3p mimic and p-MACC1).The expression levels of miR-338-3p and MACC1 mRNA in tumor tissues and cells were e-valuated by qRT-PCR.Western blotting was used to detect the protein expression levels of MACC1,B-Raf proto-oncogene(BRAF)/mitogen-activated protein kinase(MEK)/extracellular signal-regulated kinase(ERK).Clone formation test,Transwell test and scratch healing test were used to determine the cell proliferation and metastasis ability.The targeting relationship between miR-338-3p and MACC1 was analyzed by dual luciferase reporter gene assay.Nude mouse tumor model was used to evaluate the relation of miR-338-3p and MACC1.Results MACC1 was upregulated,whereas miR-338-3p was downregulated in tumor metastatic tissues and melanoma cells.miR-338-3p negatively regulates MACC1 expression.Upregulation of microRNA-338-3p and knockdown of MACC1 inhibited the proliferation,invasion and migration of A375 cells and tumor growth in mice.MACC1 promoted the malicious behaviour of A375 cells by inhibiting the MEK/ERK pathway.Conclusion Upregulation of miR-338-3p inhibits the proliferation,migration and invasion of melanoma cells by targeting MACC1 to regulate BRAF/MEK/ERK signaling pathway.关键词
miR-338-3p/MACC1/黑色素瘤/BRAF/MEK/ERK 通路/A375 细胞Key words
miR-338-3p/MACC1/melanoma/BRAF/MEK/ERK pathway/A375 cell分类
医药卫生引用本文复制引用
覃璇,龙新纯,王文娟..上调miR-338-3p通过靶向MACC1抑制恶性黑色素瘤A375细胞增殖、迁移和侵袭[J].中南医学科学杂志,2025,53(2):226-231,6.基金项目
湖南省郴州市科技计划项目(Jsyf2017039) （Jsyf2017039）
