石河子市部分流浪犬源肠球菌耐药性及耐药基因检测OA北大核心

To investigate the drug resistance and resistance gene carriage status of Enterococcus derived from stray dogs in Shihezi City,Xinjiang,this study collects 210 samples from the feces and surrounding environment of stray dogs.Enterococci were isolated,purified and identified by conventional bacterial isolation and identification methods.The drug resistance of the isolates to 14 antibiotics is determined through Kirby-Bauer disk diffusion method,and the carriage of related drug resistance genes is detected by PCR technology.A total of 163 enterococci are isolated in this study,of which 119 are from feces and 44 were from the environment.These strains include 23 Enterococcus faecalis,129 Enterococcus faecium,and 11 other Enterococcus.The results of drug sensitivity test show that Enterococcus from stray dogs have high resistance to sulfamethoxazole,clindamycin,rifampicin,tiamulin and gentamicin,and the resistant strains account for 100%,78.53%,67.48%,63.19%and 53.37%,respectively.In contrast,the resistance rates to penicillin,ampicillin and florfenicol are lower(2.45%-6.75%).It is worth noting that no strains resistant to vancomycin,tigecycline and linezolid are detected.The results of multi-drug resistance indicate that they are mainly distributed in 4-6 resistance,accounting for 69.32%(113/163),of which 6 resistance accounted for the highest proportion(25.15%),while 1 resistance account for the lowest proportion(1.23%).In addition,the drug resistance of Enterococcus in the environment is slightly more serious than that in feces,and the drug resistance of Enterococcus faecium from stray dogs is also more serious than that of Enterococcus faecalis.The results of drug resistance gene detection show that erm(B),aac(6′)/aph(2″),aph(3′)-Ⅲ,and tetM resistance genes are detected,and oxazolidinone drug-related resistance genes(optrA,cfr,poxtA),vancomycin resistance gene(van)and other drug resistance genes are not detected.In summary,stray dog shelters are likely to become a potential reservoir of multidrug-resistant bacteria,and there is a risk of epidemic related drug resistance genes.It is suggested that in the treatment of local pet dogs and stray dogs,rational drug use should be carried out according to the results of bacterial resistance,and the monitoring of bacterial resistance in stray dogs should be strengthened to reduce the risk of drug-resistant bacteria transmission.